DATASHEET
Host:
Rabbit
Target Protein:
RPS6KB1 Ser441+Thr444
Modification Site:
Ser441+Thr444
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
6198
Source:
KLH conjugated synthetic phosphopeptide derived from human RPS6KB1 around the phosphorylation site of Ser441+Thr444
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013].
PRODUCT SPECIFIC PUBLICATIONS
- Gang Lin. et al. ARID1B blocks methionine-stimulated mTOR activation to inhibit milk fat and protein synthesis in and proliferation of mouse mammary epithelial cells. J NUTR BIOCHEM. 2023 Jan;:109274Read more>>
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with RPS6KB1(Ser441+Thr444) Antibody, Unconjugated (bs-5673R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with RPS6KB1(Ser441+Thr444) Antibody, Unconjugated (bs-5673R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.