bs-5660R [Primary Antibody]
NFKB p65(Ser529) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: NFKB p65 Ser529

Modification Site: Ser529

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5970

Source: KLH conjugated synthetic phosphopeptide derived from human NFKBp65 around the phosphorylation site of Ser529

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011].

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 61


Cross Reactive Species: Human

Predicted Cross Reactive Species: Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Liu Jiawei. et al. Lactobacillus rhamnosus GR-1 Alleviates Escherichia coli-Induced Inflammation via NF-_B and MAPKs Signaling in Bovine Endometrial Epithelial Cells. Front Cell Infect Mi. 2022 Mar;0:199Read more>>
  • Chen Xiu-min. et al. Chinese Herbal Formula Huayu-Qiangshen-Tongbi Decoction Attenuates Rheumatoid Arthritis through Upregulating miR-125b to Suppress NF-_B-Induced Inflammation by Targeting CK2. J IMMUNOL RES. 2022;2022:2836128Read more>>
  • Ding Lingli. et al. Ginsenoside compound-K attenuates OVX-induced osteoporosis via the suppression of RANKL-induced osteoclastogenesis and oxidative stress. NAT PRODUCT BIOPROSP. 2023 Dec;13(1):1-12Read more>>
VALIDATION IMAGES

U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with phospho-NFKB p65 (Ser529) Antibody(bs-5660R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).