bs-5575R [Primary Antibody]
AMPK alpha 2 (Ser173) Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: AMPK alpha 2 Ser173

Specificity: Based on the high protein similarity this antibody will bind AMPK alpha 1 when phorphorylated at Ser184 and AMPK alpha 2 when phosphrylated at Ser173.

Modification Site: Ser173

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 55635562

Source: KLH conjugated synthetic phosphopeptide derived from human PRKAA2 around the phosphorylation site of Ser173

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq, Jul 2008]

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 61


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Liu T et al. MS-275 combined with cisplatin exerts synergistic antitumor effects in human esophageal squamous cell carcinoma cells. Toxicol Appl Pharmacol. 2020 May 15;395:114971.Read more>>
  • Yang T et al. Dexmedetomidine Enhances Autophagy via 2-AR/AMPK/mTOR Pathway to Inhibit the Activation of NLRP3 Inflammasome and Subsequently Alleviates Lipopolysaccharide-Induced Acute Kidney Injury. Front Pharmacol. 2020 Jun 24;11:790.Read more>>
  • Ma Y et al. IRE1 and CaMKK pathways to reveal the mechanism involved in microcystin-LR-induced autophagy in Mouseovarian cellsFood Chem Toxicol.2021 Jan;147:111911.Read more>>
  • Li Huihui. et al. Trimetazidine Ameliorates Myocardial Metabolic Remodeling in Isoproterenol-Induced Rats Through Regulating Ketone Body Metabolism via Activating AMPK and PPAR . Front Pharmacol. 2020 Aug;11:1255Read more>>
  • Jian Chen. et al. Astragalus polysaccharide alleviates transport stress-induced heart injury in newly hatched chicks via ERS-UPR-Autophagy dependent pathway. POULTRY SCIENCE. 2022 Jun;:10203Read more>>
  • W.J. Tu. et al. Osteocalcin activates lipophagy via the ADPN-AMPK/PPAR-mTOR signaling pathway in chicken embryonic hepatocyte. POULTRY SCIENCE. 2023 Nov;:103293Read more>>
VALIDATION IMAGES

Mouse kidney and heart lysates probed with AMPK alpha 2 Polyclonal Antibody, unconjugated (bs-5575R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.


Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with phospho-AMPK alpha 2 (Ser173) Antibody(bs-5575R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).