bs-5570R [Primary Antibody]
PI3KCA(Tyr317) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PI3KCA Tyr317

Modification Site: Tyr317

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5106

Swiss Prot: Q16822

Source: KLH conjugated synthetic phosphopeptide derived from human PI3KCA around the phosphorylation site of Tyr317

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 117


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang K et al. Neat1-miRNA204-5p-PI3K-AKT axis as a potential mechanism for Photodynamic Therapy treated Colitis in Mice. (2018) Photodiagnosis Photodyn Ther. Oct 29. Read more>>
  • Fan QC et al. Integrin-α5 promoted the progression of Oral Squamous Cell Carcinoma and modulated PI3K/AKT Signaling Pathway.Arch Oral Biol. 2019 May;101:85-91.Read more>>
  • Li S et al. GRP94 promotes muscle differentiation by inhibiting the PI3K/AKT/mTOR signaling pathway. J Cell Physiol. 2019 Apr 25.Read more>>
  • Li X et al. Lysine enhances the stimulation of fatty acids on milk fat synthesis via the GPRC6A-PI3K-FABP5 signaling in bovine mammary epithelial cells. J Agric Food Chem. 2019 Jun 26;67(25):7005-7015. Read more>>
  • Yu M et al. Taurine promotes milk synthesis via the GPR87-PI3K-SETD1A signaling in BMECs. J Agric Food Chem. 2019 Feb 20;67(7):1927-1936. Read more>>
  • Yang Yu et al. Vaccarin promotes proliferation of and milk synthesis in bovine mammary epithelial cells through the Prl receptor-PI3K signaling pathway. Eur J Pharmacol. 2020 Aug 5;880:173190.Read more>>
  • Wang P et al. MiR-29 regulates the function of Goat granulosa cell by targeting PTX3 via the PI3K/AKT/mTOR and Erk1/2 signaling pathways. J Steroid Biochem Mol Biol. 2020 Sep;202:105722.Read more>>
  • Kun Shi. et al. Epimedii Folium and Curculiginis Rhizoma ameliorate lipopolysaccharides-induced cognitive impairment by regulating the TREM2 signaling pathway. J Ethnopharmacol. 2022 Feb;284:114766Read more>>
  • Mingfa Ling. et al. VEGFB Promotes Myoblasts Proliferation and Differentiation through VEGFR1-PI3K/Akt Signaling Pathway. Int J Mol Sci. 2021 Jan;22(24):13352Read more>>
  • Shilin Zhang. et al. CD73-Positive Small Extracellular Vesicles Derived From Umbilical Cord Mesenchymal Stem Cells Promote the Proliferation and Migration of Pediatric Urethral Smooth Muscle Cells Through Adenosine Pathway. FRONT BIOENG BIOTECH. 2022; 10: 895998Read more>>
  • Chi, Ming. et al. TEAD4 functions as a prognostic biomarker and triggers EMT via PI3K/AKT pathway in bladder cancer. J EXP CLIN CANC RES. 2022 Dec;41(1):1-2Read more>>
  • Luo Yan. et al. Network Pharmacology and In Vivo Analysis of Dahuang-Huangqi Decoction Effectiveness in Alleviating Renal Interstitial Fibrosis. EVID-BASED COMPL ALT. 2022;2022:4194827Read more>>
  • Yusong Ge. et al. L-arginine stimulates the proliferation of mouse mammary epithelial cells and the development of mammary gland in pubertal mice by activating the GPRC6A/PI3K/AKT/mTOR signalling pathway. J ANIM PHYSIOL AN N. 2022 May 26Read more>>
  • Fang Cao. et al. Ginkgo biloba L. extract prevents steroid-induced necrosis of the femoral head by rescuing apoptosis and dysfunction in vascular endothelial cells via the PI3K/AKT/eNOS pathway. J ETHNOPHARMACOL. 2022 Jun;:115476Read more>>
  • Xin Jin. et al. GPRC6A is a key mediator of palmitic acid regulation of lipid synthesis in bovine mammary epithelial cells. CELL BIOL INT. 2022 AugRead more>>
  • Wen Li. et al. Lysine stimulates the development of the murine mammary gland at puberty via PI3K/AKT/mTOR signalling axis. J ANIM PHYSIOL AN N. 2022 AugRead more>>
  • Yu Fei. et al. Nitidine chloride induces caspase 3/GSDME-dependent pyroptosis by inhibting PI3K/Akt pathway in lung cancer. CHIN MED-UK. 2022 Dec;17(1):1-14Read more>>
  • Liu, Zhu. et al. Comprehensive transcriptomic profiling and mutational landscape of primary gastric linitis plastica. GASTRIC CANCER. 2022 Nov;:1-17Read more>>
  • Xu, Qinqin. et al. Exenatide regulates Th17/Treg balance via PI3K/Akt/FoxO1 pathway in db/db mice. MOL MED. 2022 Dec;28(1):1-14Read more>>
  • Kang Lu. et al. Micropattern Silk Fibroin Film Facilitates Tendon Repair In Vivo and Promotes Tenogenic Differentiation of Tendon Stem/Progenitor Cells through the 21/FAK/PI3K/AKT Signaling Pathway In Vitro. STEM CELLS INT. 2023 Jan 13;2023:2915826Read more>>
  • Lu, Kang. et al. N-Acetyl-L-cysteine facilitates tendon repair and promotes the tenogenic differentiation of tendon stem/progenitor cells by enhancing the integrin 5/1/PI3K/AKT signaling. BMC MOL CELL BIOL. 2023 Dec;24(1):1-13Read more>>
  • Minghui Zhang. et al. Selenomethionine promotes ANXA2 phosphorylation for proliferation and protein synthesis of myoblasts and skeletal muscle growth. J NUTR BIOCHEM. 2023 Feb;:109277Read more>>
  • Xin Liu. et al. Overexpression of YEATS2 Remodels the Extracellular Matrix to Promote Hepatocellular Carcinoma Progression via the PI3K/AKT Pathway. CANCERS. 2023 Jan;15(6):185Read more>>
  • Jingtao Zhang. et al. Ze-Qi decoction inhibits non-small cell lung cancer growth and metastasis by modulating the PI3K/Akt/p53 signaling pathway. Journal of Traditional and Complementary Medicine. 2023 MaRead more>>
  • Zhao Yin. et al. Targeting ABCB6 with nitidine chloride inhibits PI3K/AKT signaling pathway to promote ferroptosis in multiple myeloma. FREE RADICAL BIO MED. 2023 Jul;203:86Read more>>
  • Yin Qin. et al. Nobiletin attenuates monocrotaline-induced pulmonary arterial hypertension through PI3K/Akt/STAT3 pathway. J PHARM PHARMACOL. 2023 MayRead more>>
  • Fan Ling. et al. Antagonizing Effects of Chromium Against Iron-Decreased Glucose Uptake by Regulating ROS-Mediated PI3K/Akt/GLUT4 Signaling Pathway in C2C12. BIOL TRACE ELEM RES. 2023 May;:1-12Read more>>
  • o Bao. et al. Elevated retinal fibrosis in experimental myopia is involved in the activation of the PI3K/AKT/ERK signaling pathway. ARCH BIOCHEM BIOPHYS. 2023 Jul;743:109663Read more>>
  • Zhao Zhongwei. et al. Anticancer potential of Bacillus coagulans MZY531 on mouse H22 hepatocellular carcinoma cells via anti-proliferation and apoptosis induction. BMC Complementary Medicine and Therapies. 2023 Dec;23(1):1-9Read more>>
  • Chao Wang. et al. MiR-26a-5p exerts its influence by targeting EP300, a molecule known for its role in activating the PI3K/AKT/mTOR signaling pathway in CD8+tumor-infiltrating lymphocytes of colorectal cancer. CELL MOL BIOL. 2023 Nov;69(12)Read more>>
  • Jiaxin Wang. et al. Acylated Ghrelin Activates PI3K/mTOR Signaling Pathway by Promoting ThPOK Acetylation to Promote Milk Fat Synthesis in Bovine Mammary Epithelial Cells. J AGR FOOD CHEM. 2023;XXXX(XXX):XXX-XXXRead more>>
  • Xu Xiaoling. et al. NIPBL-mediated RAD21 facilitates tumorigenicity by the PI3K pathway in non-small-cell lung cancer. COMMUN BIOL. 2024 Feb;7(1):1-15Read more>>
  • Xiaoxue Fang. et al. Puerarin Induces Macrophage M2 Polarization to Exert Antinonalcoholic Steatohepatitis Pharmacological Activity via the Activation of Autophagy. J AGR FOOD CHEM. 2024;XXXX(XXX):XXX-XXXRead more>>
  • Shang Lipeng. et al. S-adenosyl-L-methionine alleviates the senescence of MSCs through the PI3K/AKT/FOXO3a signaling pathway. STEM CELLS. 2024 MaRead more>>
  • Jiaojiao Xu. et al. GluOC promotes proliferation and metastasis of TNBC through the ROCK1 signaling pathway. CANCER CELL INT. 2024; 24: 263Read more>>
  • Sishi Tang. et al. AP-1 inhibitor induces ferroptosis via the PI3K/AKT pathway in multiple myeloma cells. HELIYON. 2024 Jul;1Read more>>
  • Yu Huang. et al. Quercetin inhibits hypertonicity-induced inflammatory injury in human corneal epithelial cells via the PTEN/PI3K/AKT pathway. TISSUE CELL. 2024 Aug;89:102465Read more>>
  • Xiajie Feng. et al. Effects of heat stress on the feeding preference of yellow-feathered broilers and its possible mechanism. J THERM BIOL. 2024 Aug;:103959Read more>>
VALIDATION IMAGES

Lane 1: Mouse NIH/3T3 cell lysates; Lane 2: Mouse RAW264.7 cell lysates; Lane 3: Human Jurkat cell lysates; Lane 4: Human MCF-7 cell lysates; Lane 5: Human A431 cell lysates; Lane 6: Human Siha cell lysates probed with PI3KCA Polyclonal Antibody, Unconjugated (bs-5570R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PI3KCA(Tyr317) Polyclonal Antibody, Unconjugated (bs-5570R) at 1:200 overnight at 4°C, DAB staining.