bs-5551R-PE [Conjugated Primary Antibody]
AMPK alpha-1 (Thr198) Antibody, PE Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: AMPK alpha-1 Thr198

Modification Site: Thr198

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5562

Source: KLH conjugated synthetic phosphopeptide derived from human PRKAA1 around the phosphorylation site of Thr198

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq].

Conjugation: PE

Excitation/ Emission: 496,564nm/578nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)

Predicted Molecular Weight: 64


Cross Reactive Species: Human
Mouse
Rat
Dog

Predicted Cross Reactive Species: Cow
Sheep
Pig
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • "Liu GZ et al. Aldosterone stimulation mediates cardiac metabolism remodeling via Sirt1/AMPK signaling in canine model.Naunyn Schmiedebergs Arch Pharmacol. 2019 Mar 9."Read more>>
  • Dongmin Zou. et al. BHBA regulates the expressions of lipid synthesis and oxidation genes in sheep hepatocytes through the AMPK pathway. Res Vet Sci. 2021 Nov;140:153Read more>>
  • Wei Ruyuan. et al. Silencing TUFM Inhibits Development of Monocrotaline-Induced Pulmonary Hypertension by Regulating Mitochondrial Autophagy via AMPK/mTOR Signal Pathway. OXID MED CELL LONGEV. 2022;2022:4931611Read more>>
  • Yu Wang. et al. Seabuckthorn Reverses High-Fat-Diet-Induced Obesity and Enhances Fat Browning via Activation of AMPK/SIRT1 Pathway. NUTRIENTS. 2022 Jan;14(14):2903Read more>>
  • Jing Fan. et al. Syndecan-3 Coregulates Milk Fat Metabolism and Inflammatory Reactions in Bovine Mammary Epithelial Cells through AMPK/SIRT1 Signaling Pathway. INT J MOL SCI. 2023 Jan;24(7):6657Read more>>
  • Chunqiu Fang. et al. Tiaogan Jiejiu Tongluo Formula attenuated alcohol-induced chronic liver injury by regulating lipid metabolism in rats. J ETHNOPHARMACOL. 2023 Dec;317:116838Read more>>
  • Yixin Sun. et al. Afzelin protects against doxorubicin-induced cardiotoxicity by promoting the AMPK/SIRT1 signaling pathway. TOXICOL APPL PHARM. 2023 Sep;:116687Read more>>
  • Xiu-Li Zhang. et al. MCT1-mediated transport of valeric acid promotes porcine preimplantation embryo development by improving mitochondrial function and inhibiting the autophagic AMPK-ULK1 pathway. THERIOGENOLOGY. 2024 Sep;225:152Read more>>
  • Li Zhipeng. et al. The role of quercetin in ameliorating bleomycin-induced pulmonary fibrosis: insights into autophagy and the SIRT1/AMPK signaling pathway. MOL BIOL REP. 2024 Dec;51(1):1-1Read more>>
VALIDATION IMAGES