VALIDATION IMAGES
Lane 1: Hela cell lysates; Lane 2: Jurkat cell lysates; Lane 3: K562 cell lysates; Lane 4: Mouse Liver lysates; Lane 5: Mouse Kidney lysates probed with XPA Polyclonal Antibody, Unconjugated (bs-55224R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: XPA knockout (KO) HeLa cell lysates probed with XPA Polyclonal Antibody, Unconjugated (bs-55224R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (XPA) polyclonal Antibody, Unconjugated (bs-55224R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes.Green:GFP-RNF168 fusion protein expression for DNA damage marker.DAPI (blue, C02-04002) was used to stain the cell nuclei.
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (XPA ) polyclonal Antibody, Unconjugated (bs-55224R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes.
Lane 1: Human 293T cell lysates; Lane 2: Human HeLa cell lysates probed with XPA Polyclonal Antibody, Unconjugated (bs-55224R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.