bs-55224R [Primary Antibody]
XPA Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: XPA

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7507

Swiss Prot: P23025

Source: Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human XPA (NP_000371.1).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

This gene encodes a zinc finger protein involved in DNA excision repair. The encoded protein is part of the NER (nucleotide excision repair) complext which is responsible for repair of UV radiation-induced photoproducts and DNA adducts induced by chemical carcinogens. Mutations in this gene are associated with xeroderma pigmentosum complementation group A. Alternatively spliced transcript variants have been found for this gene.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 31kDa


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Hela cell lysates; Lane 2: Jurkat cell lysates; Lane 3: K562 cell lysates; Lane 4: Mouse Liver lysates; Lane 5: Mouse Kidney lysates probed with XPA Polyclonal Antibody, Unconjugated (bs-55224R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Hela cell lysates; Lane 2: XPA knockout (KO) HeLa cell lysates probed with XPA Polyclonal Antibody, Unconjugated (bs-55224R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (XPA) polyclonal Antibody, Unconjugated (bs-55224R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes.Green：GFP-RNF168 fusion protein expression for DNA damage marker.DAPI (blue, C02-04002) was used to stain the cell nuclei.


A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (XPA ) polyclonal Antibody, Unconjugated (bs-55224R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes.


Lane 1: Human 293T cell lysates; Lane 2: Human HeLa cell lysates probed with XPA Polyclonal Antibody, Unconjugated (bs-55224R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.