DATASHEET
Host:
Rabbit
Target Protein:
VAT1
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
10493
Swiss Prot:
Q99536
Source:
A synthetic peptide of human VAT1.
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
Synaptic vesicles are responsible for regulating the storage and release of neurotransmitters in the nerve terminal. The protein encoded by this gene is an abundant integral membrane protein of cholinergic synaptic vesicles and is thought to be involved in vesicular transport. It belongs to the quinone oxidoreductase subfamily of zinc-containing alcohol dehydrogenase proteins.
VALIDATION IMAGES
Lane 1: K562 cell lysates; Lane 2: U-87MG cell lysates probed with VAT1 Polyclonal Antibody, Unconjugated (bs-55221R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 293T cell lysates; Lane 2: VAT1 knockout (KO) 293T cell lysates probed with VAT1 Polyclonal Antibody, Unconjugated (bs-55221R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.