bs-55203R [Primary Antibody]
SMARCA4 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SMARCA4

Specificity: KO-Validated

Immunogen Range: 30-130/1647


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6597

Swiss Prot: P51532

Source: A synthetic peptide corresponding to a sequence within amino acids 30-130 of human SMARCA4 (NP_003063.2).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 181


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: 293T cell lysates; Lane 2: SMARCA4 knockout (KO) 293T cell lysates probed with SMARCA4 Polyclonal Antibody, Unconjugated (bs-55203R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: OVCAR3 cell lysates; Lane 2: HeLa cell lysates probed with SMARCA4 Polyclonal Antibody, Unconjugated (bs-55203R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SMARCA4 Polyclonal Antibody, Unconjugated (bs-55203R) at 1:100 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SMARCA4 Polyclonal Antibody, Unconjugated (bs-55203R) at 1:100 overnight at 4°C, DAB staining.