bs-55186R [Primary Antibody]
RALBP1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: RALBP1

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 10928

Swiss Prot: Q15311

Source: Recombinant fusion protein containing a sequence corresponding to amino acids 396-655 of human RALBP1 (NP_006779.1).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 76kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Hela cell lysates; Lane 2: RALBP1 knockout (KO) HeLa cell lysates probed with RALBP1 Polyclonal Antibody, Unconjugated (bs-55186R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Human A673 cell lysates; Lane 2: Mouse spleen lysates; Lane 3: Mouse lung lysates probed with RALBP1 Polyclonal Antibody, Unconjugated (bs-55186R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RALBP1 Polyclonal Antibody, Unconjugated (bs-55186R) at 1:100 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RALBP1 Polyclonal Antibody, Unconjugated (bs-55186R) at 1:100 overnight at 4°C, DAB staining.