bs-55138R [Primary Antibody]
N-Cadherin Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: N-Cadherin

Specificity: KO-Validated

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1000

Swiss Prot: P19022

Source: A synthetic peptide corresponding to a sequence within amino acids 750-850 of human N-Cadherin (NP_001783.2).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 97/99


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Jing Liu. et al. Exosomes Promote Atherosclerosis Progression by Regulating Circ_100696/miR-503-5p/PAPPA Axis-Mediated Vascular Smooth Muscle Cells Proliferation and Migration. INT HEART J. 2023 Sep;64(5):918-927Read more>>
VALIDATION IMAGES

Lane 1: Hela whole cell lysates; Lane 2: Hela membrane protein extract probed with N-Cadherin Polyclonal Antibody, Unconjugated (bs-55138R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Hela cell lysates; Lane 2: N-Cadherin knockout (KO) HeLa cell lysates probed with N-Cadherin Polyclonal Antibody, Unconjugated (bs-55138R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Hela cell lysates; Lane 2: A549 cell lysates; Lane 3: 293T cell lysates; Lane 4: Mouse Liver lysates; Lane 5: Mouse Brain lysates; Lane 6: Mouse Heart lysates; Lane 7: Rat Liver lysates; Lane 8: Rat Heart lysates probed with N-Cadherin Polyclonal Antibody, Unconjugated (bs-55138R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.