bs-5512R [Primary Antibody]
NFKB1 (Ser337) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: NFKB1 Ser337

Modification Site: Ser337

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4790

Source: KLH conjugated synthetic phosphopeptide derived from human NF KappaB p105 around the phosphorylation site of Ser337

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009].

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 48/105


Cross Reactive Species: Human
Mouse
Others

Predicted Cross Reactive Species: Cow
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Lei Zhao. et al. Proteomic analysis reveals the molecular mechanism of Hippophae rhamnoides polysaccharide intervention in LPS-induced inflammation of IPEC-J2 cells in piglets. Int J Biol Macromol. 2020 Dec;164:3294Read more>>
  • Chen Bai. et al. Ac2?26 alleviates hepatic ischemia?reperfusion injury based on inhibiting the positive feedback loop of HMGB1/TLR4/NF?B/neutrophils. EXP THER MED. 2022 Nov;24(5):1-1Read more>>
  • Houpan Song. et al. Traditional Chinese Medicine prescription Huang-Qi-Jian-Zhong-Tang ameliorates indomethacin-induced duodenal ulcers in rats by affecting NF-B and STAT signaling pathways. BIOMED PHARMACOTHER. 2022 Dec;156:113866Read more>>
  • Lisha Peng. et al. miR-128-3p increases the radiosensitivity in nasopharyngeal carcinoma via regulating vascular endothelial growth factor C. PATHOL RES PRACT. 2023 Aug;:154768Read more>>
  • Qifang Sun. et al. Astragaloside IV ameliorates indomethacin-induced intestinal inflammation in rats through inhibiting the activation of NLRP3 inflammasome. INT IMMUNOPHARMACOL. 2024 Jun;135:112281Read more>>
  • Yaping Yu. et al. Dendrobium nobile active ingredient Dendrobin A against hepatocellular carcinoma via inhibiting nuclear factor kappa-B signaling. BIOMED PHARMACOTHER. 2024 Aug;177:117013Read more>>
  • Chengzhi Yuan. et al. Atractylenolide I Alleviates Indomethacin-Induced Gastric Ulcers in Rats by Inhibiting NLRP3 Inflammasome Activation. J AGR FOOD CHEM. 2024;XXXX(XXX):XXX-XXXRead more>>
  • Zumureti Abudukeyimu. et al. Early growth response factor 3 regulates coronary atherosclerosis through the NF-B signaling pathway and VEGF expression. AM J MED SCI. 2024 JulRead more>>
VALIDATION IMAGES

Antigen: bs-5512P, 0.2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; \nTMB staining; Read the data in Microplate Reader by 450nm\n


Raji cell lysates probed with NFKB1 (Ser337) Polyclonal Antibody, Unconjugated (bs-5512R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


A549 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with NFKB1 (Ser337) Polyclonal Antibody(bs-5512R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).


Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NFKB1 (Ser337) Polyclonal Antibody, Unconjugated (bs-5512R) at 1:400 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human rectal cancer ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NFKB1 (Ser337) Polyclonal Antibody, Unconjugated (bs-5512R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NFKB1 (Ser337) Polyclonal Antibody, Unconjugated (bs-5512R) at 1:200 overnight at 4°C, DAB staining.