DATASHEET
Host:
Rabbit
Target Protein:
HMGCR
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
3156
Swiss Prot:
P04035
Source:
Recombinant fusion protein containing a sequence corresponding to amino acids 629-888 of human HMGCR (NP_000850.1).
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
VALIDATION IMAGES
Lane 1: 293T cell lysates; Lane 2: HMGCR knockout (KO) 293T cell lysates probed with HMGCR Polyclonal Antibody, Unconjugated (bs-55100R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: HepG2 cell lysates; Lane 2: Hela cell lysates probed with HMGCR Polyclonal Antibody, Unconjugated (bs-55100R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.