DATASHEET
Host:
Rabbit
Target Protein:
HINT1
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
3094
Swiss Prot:
P49773
Source:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-126 of human HINT1 (NP_005331.1).
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
This gene encodes a protein that hydrolyzes purine nucleotide phosphoramidates substrates, including AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif. This gene is considered a tumor suppressor gene. In addition, mutations in this gene can cause autosomal recessive neuromyotonia and axonal neuropathy. There are several related pseudogenes on chromosome 7. Several transcript variants have been observed.
VALIDATION IMAGES
Lane 1: Hela cell lysates; Lane 2: HINT1 knockout (KO) HeLa cell lysates probed with HINT1 Polyclonal Antibody, Unconjugated (bs-55097R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 22RV1 cell lysates; Lane 2: Hela cell lysates; Lane 3: H460 cell lysates; Lane 4: K562 cell lysates; Lane 5: Mouse Kidney lysates; Lane 6: Mouse Liver lysates; Lane 7: Rat Kidney lysates probed with HINT1 Polyclonal Antibody, Unconjugated (bs-55097R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.