bs-55097R [Primary Antibody]
HINT1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: HINT1

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3094

Swiss Prot: P49773

Source: Recombinant fusion protein containing a sequence corresponding to amino acids 1-126 of human HINT1 (NP_005331.1).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

This gene encodes a protein that hydrolyzes purine nucleotide phosphoramidates substrates, including AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif. This gene is considered a tumor suppressor gene. In addition, mutations in this gene can cause autosomal recessive neuromyotonia and axonal neuropathy. There are several related pseudogenes on chromosome 7. Several transcript variants have been observed.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 13kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Hela cell lysates; Lane 2: HINT1 knockout (KO) HeLa cell lysates probed with HINT1 Polyclonal Antibody, Unconjugated (bs-55097R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: 22RV1 cell lysates; Lane 2: Hela cell lysates; Lane 3: H460 cell lysates; Lane 4: K562 cell lysates; Lane 5: Mouse Kidney lysates; Lane 6: Mouse Liver lysates; Lane 7: Rat Kidney lysates probed with HINT1 Polyclonal Antibody, Unconjugated (bs-55097R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.