bs-55095R [Primary Antibody]
HELLS Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: HELLS

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3070

Swiss Prot: Q9NRZ9

Source: Recombinant fusion protein containing a sequence corresponding to amino acids 719-838 of human HELLS (NP_060533.2).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

This gene encodes a lymphoid-specific helicase. Other helicases function in processes involving DNA strand separation, including replication, repair, recombination, and transcription. This protein is thought to be involved with cellular proliferation and may play a role in leukemogenesis. Alternatively spliced transcript variants encoding different isoforms have been identified.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 14kDa/36-39kDa/81-97kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HELLS Polyclonal Antibody, Unconjugated (bs-55095R) at 1:100 overnight at 4°C, DAB staining.


Lane 1: MCF-7 cell lysates; Lane 2: A549 cell lysates; Lane 3: 293T cell lysates; Lane 4: HepG2 cell lysates; Lane 5: Mouse thymus lysates probed with HELLS Polyclonal Antibody, Unconjugated (bs-55095R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Hela cell lysates; Lane 2: HELLS knockout (KO) HeLa cell lysates probed with HELLS Polyclonal Antibody, Unconjugated (bs-55095R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.