bs-55078R [Primary Antibody]
FSCN1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: FSCN1

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6624

Swiss Prot: Q16658

Source: Recombinant fusion protein containing a sequence corresponding to amino acids 394-493 of human FSCN1 (NP_003079.1).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 54kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with FSCN1 Polyclonal Antibody, Unconjugated (bs-55078R) at 1:100 overnight at 4°C, DAB staining.


Lane 1: Hela cell lysates; Lane 2: FSCN1 knockout (KO) HeLa cell lysates probed with FSCN1 Polyclonal Antibody, Unconjugated (bs-55078R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: U251MG cell lysates; Lane 2: Hela cell lysates; Lane 3: NCI-H460 cell lysates; Lane 4: HepG2 cell lysates; Lane 5: SH-SY5Y cell lysates; Lane 6: Mouse Brain lysates; Lane 7: Mouse spinal cord lysates probed with FSCN1 Polyclonal Antibody, Unconjugated (bs-55078R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Rat skin cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (FSCN1) polyclonal Antibody, Unconjugated (bs-55078R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.


Human skin cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (FSCN1) polyclonal Antibody, Unconjugated (bs-55078R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.