bs-55043R [Primary Antibody]
CHD1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CHD1

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1105

Swiss Prot: O14646

Source: A synthetic peptide corresponding to a sequence within amino acids 1600 to the C-terminus of human CHD1 (NP_001261.2).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 196kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CHD1 Polyclonal Antibody, Unconjugated (bs-55043R) at 1:100 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human liver damage; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CHD1 Polyclonal Antibody, Unconjugated (bs-55043R) at 1:100 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CHD1 Polyclonal Antibody, Unconjugated (bs-55043R) at 1:100 overnight at 4°C, DAB staining.


Lane 1: 293T cell lysates; Lane 2: CHD1 knockout (KO) 293T cell lysates probed with CHD1 Polyclonal Antibody, Unconjugated (bs-55043R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Hela cell lysates; Lane 2: Jurkat cell lysates; Lane 3: K562 cell lysates probed with CHD1 Polyclonal Antibody, Unconjugated (bs-55043R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.