bs-55039R
[Primary Antibody]
CD44 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: CD44
Specificity: KO-Validated
Immunogen Range: 148-247/742
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 960
Swiss Prot: P16070
Source: Recombinant fusion protein containing a sequence corresponding to amino acids 148-247 of human CD44 (NP_001189486.1).
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
Size: 100ul
Concentration: 1ug/ul
Predicted Molecular Weight: 3kDa/15kDa/32-53kDa/73-81kDa
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Yu He. et al. Single-Cell RNA Sequencing Unravels Distinct Tumor Microenvironment of Different Components of Lung Adenocarcinoma Featured as Mixed Ground-Glass Opacity. FRONT IMMUNOL. 2022; 13: 903513Read more>>
- Juan Cen. et al. A Water-Soluble Quercetin Conjugate with Triple Targeting Exerts Neuron-Protective Effect on Cerebral Ischemia by Mitophagy Activation. ADV HEALTHC MATER. 2022 Sep;:2200817Read more>>
- Ma Caiyun. et al. Characteristics and Multilineage Differentiation of a Novel Type of Pulmonary Mesenchymal Stem Cells Derived from Goose Embryos. BRAZ ARCH BIOL TECHN. 2024 Apr;67:e24230139Read more>>
VALIDATION IMAGES
A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (CD44) polyclonal Antibody, Unconjugated (bs-55039R) 1:200, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Raw264.7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (CD44) polyclonal Antibody, Unconjugated (bs-55039R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (CD44) polyclonal Antibody, Unconjugated (bs-55039R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (CD44) polyclonal Antibody, Unconjugated (bs-55039R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Hela cell lysates; Lane 2: CD44 knockout (KO) HeLa cell lysates probed with CD44 Polyclonal Antibody, Unconjugated (bs-55039R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse spleen cell lysates probed with CD44 Polyclonal Antibody, Unconjugated (bs-55039R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human A549 cell lysates; Lane 2: Human U2os cell lysates; Lane 3: Human MDA-MB-231 cell lysates; Lane 4: Human Hela cell lysates probed with CD44 Polyclonal Antibody, Unconjugated (bs-55039R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Hela cell (Control) lysates; Lane 2: CD44 knockout (KO) Hela cell lysates probed with CD44 Polyclonal Antibody, Unconjugated (bs-55039R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Hela cell lysates; Lane 2: Human Panc-1 cell lysates; Lane 3: Human Huvec cell lysates; Lane 4: Human A549 cell lysates probed with CD44 Polyclonal Antibody, Unconjugated (bs-55039R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.