DATASHEET
Host:
Rabbit
Target Protein:
CAST
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
831
Swiss Prot:
P20810
Source:
A synthetic peptide corresponding to a sequence within amino acids 550-650 of human CAST (NP_001177371.1).
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described.
VALIDATION IMAGES
Lane 1: Hela cell lysates; Lane 2: CAST knockout (KO) HeLa cell lysates probed with CAST Polyclonal Antibody, Unconjugated (bs-55034R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: A549 cell lysates; Lane 2: SW480 cell lysates; Lane 3: MCF-7 cell lysates; Lane 4: HepG2 cell lysates; Lane 5: Mouse Lung lysates; Lane 6: Rat Liver lysates probed with CAST Polyclonal Antibody, Unconjugated (bs-55034R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.