VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded Rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Caspase-3 Polyclonal Antibody, Unconjugated (bs-55032R) at 1:100 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Caspase-3 Polyclonal Antibody, Unconjugated (bs-55032R) at 1:100 overnight at 4°C, DAB staining.
Lane 1: Jurkat cell lysates; Lane 2: Jurkat cell lysates probed with Caspase-3 Polyclonal Antibody, Unconjugated (bs-55032R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 293T cell lysates; Lane 2: Caspase-3 knockout (KO) 293T cell lysates probed with Caspase-3 Polyclonal Antibody, Unconjugated (bs-55032R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human HL-60 cell lysates; Lane 2: Human MOLT4 cell lysates; Lane 3: Human K562 cell lysates probed with Caspase-3 Polyclonal Antibody, Unconjugated (bs-55032R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
25 ug total protein per lane of various lysates (see on figure) probed with Caspase-3 polyclonal antibody, unconjugated (bs-55032R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.