bs-55032R
[Primary Antibody]
Caspase-3 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Caspase-3
Immunogen Range: 55-160/277
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 836
Swiss Prot: P42574
Source: Recombinant fusion protein containing a sequence corresponding to amino acids 55-160 of human Caspase-3 (NP_004337.2).
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
Size: 100ul
Concentration: 1ug/ul
Predicted Molecular Weight: 31
Cross Reactive Species:
Human
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Shaohua Hou. et al. Design, synthesis and biological evaluation of 1H-indazole derivatives as novel ASK1 inhibitors. Eur J Med Chem. 2021 Aug;220:113482Read more>>
- Vlasta Demeckova. et al. 5-Fluorouracil Treatment of CT26 Colon Cancer Is Compromised by Combined Therapy with IMMODIN. INT J MOL SCI. 2022 Jan;23(12):6374Read more>>
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded Rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Caspase-3 Polyclonal Antibody, Unconjugated (bs-55032R) at 1:100 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Caspase-3 Polyclonal Antibody, Unconjugated (bs-55032R) at 1:100 overnight at 4°C, DAB staining.
Lane 1: Jurkat cell lysates; Lane 2: Jurkat cell lysates probed with Caspase-3 Polyclonal Antibody, Unconjugated (bs-55032R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 293T cell lysates; Lane 2: Caspase-3 knockout (KO) 293T cell lysates probed with Caspase-3 Polyclonal Antibody, Unconjugated (bs-55032R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human HL-60 cell lysates; Lane 2: Human MOLT4 cell lysates; Lane 3: Human K562 cell lysates probed with Caspase-3 Polyclonal Antibody, Unconjugated (bs-55032R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
25 ug total protein per lane of various lysates (see on figure) probed with Caspase-3 polyclonal antibody, unconjugated (bs-55032R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Caspase-3 Polyclonal Antibody, Unconjugated (bs-55032R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Caspase-3 Polyclonal Antibody, Unconjugated (bs-55032R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Caspase-3 Polyclonal Antibody, Unconjugated (bs-55032R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.