bs-55011R [Primary Antibody]
ANXA5 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ANXA5

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 308

Swiss Prot: P08758

Source: Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human ANXA5 (NP_001145.1).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 35kDa


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: 293T cell lysates; Lane 2: ANXA5 knockout (KO) 293T cell lysates probed with ANXA5 Polyclonal Antibody, Unconjugated (bs-55011R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: HepG2 cell lysates; Lane 2: Hela cell lysates; Lane 3: DU145 cell lysates; Lane 4: PC-3 cell lysates; Lane 5: Mouse Lung lysates probed with ANXA5 Polyclonal Antibody, Unconjugated (bs-55011R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.