bs-5450R [Primary Antibody]
ICAM1(Tyr512) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ICAM1 Tyr512

Modification Site: Tyr512

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3383

Swiss Prot: P05362

Source: KLH conjugated synthetic phosphopeptide derived from human ICAM1 around the phosphorylation site of Tyr512

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

ICAM proteins are ligands for the leukocyte adhesion LFA-1 protein (Integrin alpha-L/beta-2). Subcellular Located at Membrane; single-pass type I membrane protein. Tissue Specificified Expressed at low level on a subpopulation of lymphocytes, macrophages, and endothelial cells, but is strongly induced on these cells, and on fibroblasts and epithelial cells. [PTM] Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis (By similarity). It belongs to the immunoglobulin superfamily. ICAM family.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 55


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Human MCF-7 lysates probed with phospho-ICAM1 (Tyr512) Polyclonal Antibody, Unconjugated (bs-5450R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.