bs-5367R [Primary Antibody]
GSK-3 Beta(Ser21+Ser29) Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GSK-3 Beta Ser21+Ser29

Modification Site: Ser21+Ser29

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2932

Source: KLH conjugated synthetic phosphopeptide derived from human GSK-3 Beta around the phosphorylation site of Ser21+Ser29

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Glycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. GSK3 has been implicated in fundamental cell processes such as cell fate determination, metabolism, transcriptional control and oncogenesis. Two isoforms, alpha (GSK3A; OMIM 606784) and beta, show a high degree of amino acid homology within their catalytic domains. GSK3B is involved in energy metabolism, neuronal cell development and body pattern formation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 46


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig
Horse
Chicken
GuineaPig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • J Song. et al. MSCs reduce airway remodeling in the lungs of asthmatic rats through the Wnt/-catenin signaling pathway.. Eur Rev Med Pharmaco. 2020 Nov;24(21):11199-11211Read more>>
  • Liu Jiayi. et al. Lithium Chloride Promotes Endogenous Synthesis of CLA in Bovine Mammary Epithelial Cells. BIOL TRACE ELEM RES. 2023 Apr;:1-14Read more>>
  • Jiaqi Li. et al. Gold Nanoparticle Delivery of Glut1 siRNA Facilitates Glucose Starvation Therapy in Lung Cancer. CHEMBIOCHEM. 2024 Apr;:e202400239Read more>>
VALIDATION IMAGES

Lane 1: Mouse Muscle lysates; Lane 2: Mouse Liver lysates; Lane 3: Mouse Cerebrum lysates; Lane 4: Mouse Pancreas lysates; Lane 5: Mouse Thymus lysates; Lane 6: Rat Muscle lysates; Lane 7: Rat Cerebrum lysates; Lane 8: Rat Pancreas lysates; Lane 9: Rat Thymus lysates; Lane 10: Human Hela cell lysates; Lane 11: Human MCF-7 cell lysates; Lane 12: Human A431 cell lysates probed with GSK-3 Beta Polyclonal Antibody, Unconjugated (bs-5367R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (phospho-GSK-3 Beta (Ser21+Ser29)) polyclonal Antibody, Unconjugated (bs-5367R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded (human pancreas ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (GSK3 Beta (Ser21+Ser29) ) Polyclonal Antibody, Unconjugated (bs-5367R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (GSK3 Beta (Ser21+Ser29) ) Polyclonal Antibody, Unconjugated (bs-5367R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.