bs-5337R [Primary Antibody]
EIF4EBP1(Ser112) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: E4EBP1 Ser112

Modification Site: Ser112

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1978

Source: KLH conjugated synthetic phosphopeptide derived from mouse 4EBP1 around the phosphorylation site of Ser112

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Regulates eIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 13


Predicted Cross Reactive Species: Human
Mouse
Rat
Dog
Cow
Horse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Kidney tissue lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human MCF-7 cell lysates probed with phospho-EIF4EBP1 (Ser111) Polyclonal Antibody, Unconjugated (bs-5337R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.