Host: Rabbit
Target Protein: H3K56ac
Clonality: Polyclonal
Isotype: Not Determined
Entrez Gene: 8350
Swiss Prot: P68431
Source: Polyclonal antibody raised in rabbit against the region of histone H3 containing the acetylated lysine 56 (H3K56ac), using a KLH-conjugated synthetic peptide
Purification: Affinity purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Size: 50ug
Concentration: 0.6ug/ul
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
To test the cross reactivity of the H3K56ac Polyclonal Antibody (bs-53145R), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K56. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.
To determine the titer of the antibody, an ELISA was performed using a serial dilution of H3K56ac Polyclonal Antibody (bs-53145R) in antigen-coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:15,300.
ChIP was performed on sheared chromatin from 1.5 million HeLaS3 cells using 5 μg of H3K56ac Polyclonal Antibody (bs-53145R) as described previously. The IP’d DNA was subsequently analyzed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure shows the enrichment along the complete sequence and a 1 Mb region of the X-chromosome (fig A and B) and in genomic regions of chromosome 12 and 3, surrounding the GAPDH and EIF4A2 positive control genes.