HeLa cells were stained with the Bioss antibody against WDR5 (Cat. No. bs-53101R) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the WDR5 antibody (left) diluted 1:1,000 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.


Nuclear extracts from HeLa cells (20 μg) were analyzed by Western blot using the Bioss antibody against WDR5 (Cat. No. bs-53101R) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.


ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Bioss antibody against WDR5 (Cat. No. bs-53101R) as described above. The IP’d DNA was subsequently analyzed on an Illumina HiSeq. Library preparation, cluster generation, and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. The figure shows the enrichment along the complete sequence and a 2 Mb region of human chromosome 1 (fig A and B), and in two genomic regions surrounding the RPL41 and RPS4X positive control genes (fig C and D).