bs-53045R [Primary Antibody]
TAF1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TAF1

Clonality: Polyclonal

Isotype: Not Determined

Entrez Gene: 6872

Swiss Prot: P21675

Source: Polyclonal antibody raised in rabbit against TAF1 using a KLH-conjugated synthetic peptide.

Purification: Affinity purification

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Largest component and core scaffold of the TFIID basal transcription factor complex (PubMed:25412659, PubMed:27007846). Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity (PubMed:25412659). Essential for progression of the G1 phase of the cell cycle (PubMed:11278496, PubMed:15053879, PubMed:2038334, PubMed:8450888, PubMed:8625415, PubMed:9660973, PubMed:9858607). Exhibits histone acetyltransferase activity towards histones H3 and H4 (PubMed:15870300).

Size: 100ug

Concentration: 1.15μg/μl

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Immunohistochemistry of TAF1 antibody. Tissue: prostate. Fixation: formalin fixed paraffin embedded. Primary antibody: TAF1 (bs-53045R) at 10 μg/mL for 1h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 dilution for 45 min at RT. Staining: TAF1 as precipitated red signal with hematoxylin purple nuclear counterstain.


Western blot using the TAF1 antibody (bs-53045R) to detect TAF1 in HeLa nuclear extract (arrowhead). The membrane was probed with the primary antibody at dilutions of 1:100 (lane 1), 1:250 (lane 2), 1: 500 (Lane 3) and 1:1,000 (Lane 4). The identity of the bands at ~95 kDa is unknown but may be degraded TAF1.