Host: Rabbit
Target Protein: H3K4me3
Clonality: Polyclonal
Isotype: Not Determined
Entrez Gene: 8350
Swiss Prot: P68431
Source: Polyclonal antibody raised in rabbit against the region of histone H3 containing the trimethylated lysine 4 (H3K4me3), using a KLH-conjugated synthetic peptide
Purification: Whole antiserum from rabbit containing 0.05% azide
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Size: 100ul
Concentration: Not Determined
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
A Dot Blot analysis was performed to test the cross reactivity of H3K4me3 antibody (bs-53034R) with peptides containing other modifications and unmodified sequences of histone H3 and H4. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:2,000. The figure shows a high specificity of the antibody for the modification of interest.
Western blot was performed using histone extracts from HeLa cells (15 μg) and H3K4me3 antibody (bs-53034R) diluted 1:500 in TBS-Tween containing 5% skimmed milk. A molecular weight marker (in kDa) is shown on the left, the position of the protein of interest is shown on the right.
HeLa cells were stained with the H3K4me3 antibody (bs-53034R) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were labelled with the H3K4me3 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.