bs-5277R [Primary Antibody]
CDK2 (Thr14) Polyclonal Antibody
www.biossusa.com
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DATASHEET

Host: Rabbit

Target Protein: CDK2 Thr14

Modification Site: Thr14

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1017

Source: KLH conjugated synthetic phosphopeptide derived from human CDK2 around the phosphorylation site of Thr14 [EG(p-T)YG]

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. cdk2 is a cell cycle protein closely related to Cdc2 (cdk1) that has proved useful as a marker of proliferation. cdk2 binds cyclin type A and E proteins and controls progression into S-phase.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 34


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Dog
Cow
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Liao, Fang-Hsuean, et al. "T cell proliferation and adaptive immune responses are critically regulated by protein phosphatase 4." Cell Cycle (2016).Read more>>
  • Tao Wang. et al. ABRO1 arrests postnatal cardiomyocyte proliferation and myocardial repair by blocking N6-methyladenosine methylation and expression of PSPH. MOL THER. 2023 JaRead more>>
VALIDATION IMAGES

Molt4 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature.Cells were then stained with CDK2 (Thr14) Polyclonal Antibody(bs-R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).