DATASHEET
Host:
Rabbit
Target Protein:
CREB-1Ser142
Modification Site:
Ser142
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
1385
Source:
KLH conjugated synthetic phosphopeptide derived from human CREB-1 around the phosphorylation site of Ser142 [DL(p-S)SD]
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
The ATF/CREB family consists of transcription factors that function through binding to the cAMP responsive element (CRE) palindromic octanucleotide, TGACCTCA. The best characterized members of this gene family include CREB-1, CREB-2, ATF-1,ATF-2,ATF-3and ATF-4. these transcription factors share highly-related COOH terminal leucine zipper demerization and basic DNA bindings but are highly divergent in their amino terminal domains. Although each of the ATF/CREB proteins bind CREs in their homodimeric form, in cerain instances they also bind as heterodimers, both within the ATF/CREB family and with members of the AP-1 transcription factor family. It has recentlybeen shown that protein kinase A-mediated CREB phosphorylation results in its binding to a 265kDa nuclear protein designated CBP (CREB-binding protein), which may reprecent a CREB co-activator.
PRODUCT SPECIFIC PUBLICATIONS
- Xiaoyue Guan. et al. The possible role of Gremlin1 in inflammatory apical periodontitis. ARCH ORAL BIOL. 2023 Nov;:105848Read more>>
VALIDATION IMAGES
Hela cells were fixed with 70% ice-cold methanol overnight at 4℃,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CREB-1(Ser142) Polyclonal Antibody(bs-5269R) at 1:50dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), no antibody control control (blue), secondary antibody only (light blue) and isotype control (orange).