bs-5178R [Primary Antibody]
Chondroitin Sulfate Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Chondroitin Sulfate

Clonality: Polyclonal

Isotype: IgG

Source: Chondroitin Sulfate

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Chondroitin sulphate proteoglycans (CSPGs) are upregulated in the central nervous system after injury, specifically around the lesion site where the glial scar forms. CSPGs have been shown to be closely involved in this neuronal growth inhibition, specifically through their sugar chains.Chondroitin sulphate proteoglycans may also play a role in the repair of spinal cord injury.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IF(ICC)(1:50-200)

Predicted Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Leixin Wei. et al. Chondroitin synthase\3 regulates nucleus pulposus degeneration through actin\induced YAP signaling. Faseb J. 2020 Dec;34(12):16581-166Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse lymph node; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Chondroitin Sulfate Polyclonal Antibody, Unconjugated (bs-5178R) at 1:400 overnight at 4°C, followed by secondary and DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Chondroitin Sulfate Polyclonal Antibody, Unconjugated (bs-5178R) at 1:400 overnight at 4°C, followed by secondary and DAB staining.