bs-5155R [Primary Antibody]
LSP1 (Ser204) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: LSP1 Ser204

Specificity: This phosphorylation site is homologous to that of Ser195 in Mouse, Ser196 in Rat and Ser228 in Bovine.

Modification Site: Ser204

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4046

Swiss Prot: P33241

Source: KLH conjugated synthetic phosphopeptide derived from human LSP1 around the phosphorylation site of Ser204

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

May play a role in mediating neutrophil activation and chemotaxis. This gene encodes an intracellular F-actin binding protein. The protein is expressed in lymphocytes, neutrophils, macrophages, and endothelium and may regulate neutrophil motility, adhesion to fibrinogen matrix proteins, and transendothelial migration. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 37


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

L1 human colon carcinoma lysates, L2 mouse brain lystaes probed (bs-5155R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 52kDa.\n


Mouse urinary bladder lysates probed with LSP1 (Ser204) Polyclonal Antibody, Unconjugated (bs-5155R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


25 ug total protein per lane of various lysates (see on figure) probed with phospho-LSP1 (Ser204) polyclonal antibody, unconjugated (bs-5155R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.