Host: Rabbit
Target Protein: Acrosin
Immunogen Range: 201-300/421
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 49
Swiss Prot: P10323
Source: KLH conjugated synthetic peptide derived from human Acrosin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 33/44
Cross Reactive Species:
Human
Mouse
Rat
Goat
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
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- Deng, Shou‐Long, et al. "Melatonin promotes development of haploid germ cells from early developing spermatogenic cells of Suffolk sheep under in vitro condition." Journal of Pineal Research (2016).Read more>>
- Deng et al. Melatonin up-regulates the expression of the GATA-4 transcription factor and increases testosterone secretion from Leydig cells through RORα signaling in anin vitrogoat spermatogonial stem cell differentiation culture system. (2017) Oncotarget. 8:110592-110605Read more>>
- Liu Y et al. Fluoride interferes with sperm fertilizing ability via downregulated SPAM1, ACR and PRSS21 expression in rat epididymis. J Agric Food Chem. 2019 May 8;67(18):5240-5249. Read more>>
- Yu K et al. In-vitro differentiation of early pig spermatogenic cells to haploid germ cells. Mol Hum Reprod. 2019 Jul 22. pii: gaz043.Read more>>
- Li Y et al. Seminal Plasma Proteome as an Indicator of Sperm Dysfunction and Low Sperm Motility in Chickens. Mol Cell Proteomics. 2020 Jun;19(6):1035-1046.Read more>>
- Ru Yu. et al. ATF6 deficiency damages the development of spermatogenesis in male Atf6 knockout mice. 2021 Dec 13Read more>>
- Zhang, Guohui. et al. Deficiency of cancer/testis antigen gene CT55 causes male infertility in humans and mice. CELL DEATH DIFFER. 2022 Dec;:1-15Read more>>
Formalin-fixed and paraffin embedded rat testis labeled with Anti-Acrosin Polyclonal Antibody, Unconjugated (bs-5151R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
HUVEC cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Acrosin Polyclonal Antibody(bs-5151R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Acrosin) polyclonal Antibody, Unconjugated (bs-5151R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Acrosin Polyclonal Antibody(bs-5151R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).