bs-4989R
[Primary Antibody]
BDNF Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: BDNF
Immunogen Range: 151-247/247
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 627
Swiss Prot: P23560
Source: KLH conjugated synthetic peptide derived from human BDNF
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 13/27
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Pig
Horse
Rabbit
Guinea Pig
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Zhao, Tianyun, et al. "Ketamine administered to pregnant rats in the second trimester causes long-lasting behavioral disorders in offspring." Neurobiology of Disease (2014).Read more>>
- He, Xiaolie, et al. "Targeting the Endocannabinoid/CB1 Receptor System For Treating Major Depression Through Antidepressant Activities of Curcumin and Dexanabinol-Loaded Solid Lipid Nanoparticles." Cellular Physiology and Biochemistry 42.6 (2017): 2281-2294.Read more>>
- Nader, Manar A., et al. "Sitagliptin enhances the neuroprotective effect of pregabalin against pentylenetetrazole-induced acute epileptogenesis in mice: Implication of oxidative, inflammatory, apoptotic and autophagy pathways." Neurochemistry International(2017).Read more>>
- Xing et al. Non-viral liposome-mediated transfer of brain-derived neurotrophic factor across the blood-brain barrier. (2016) Neural.Regen.Re. 11:617-22Read more>>
- Zabłocka et al. Neurotrophic Activity of Cultured Cell Line U87 is Up-Regulated by Proline-Rich Polypeptide Complex and Its Constituent Nonapeptide. (2015) Cell.Mol.Neurobiol. 35:977-86Read more>>
- Xu J. Y. et al. The Antidepressant-Like Effect of Vanillin Aroma Involves Serum Magnesium and Brain BDNF. (2018)Neuropsychiatry (London) Volume 8, Issue 4Read more>>
- Sheen JM et al. Young rats with increased circulatory asymmetric dimethylarginine exhibited spatial deficit and alterations in dorsal hippocampus brain-derived neurotrophic factor and asymmetric dimethylarginine: Effects of melatonin. Int J Dev Neurosci. 2019 Sep 21;78:83-89. Read more>>
- Liu P et al. Harmine Ameliorates Cognitive Impairment by Inhibiting NLRP3 Inflammasome Activation and Enhancing the BDNF/TrkB Signaling Pathway in STZ-Induced Diabetic Rats. Front Pharmacol. 2020 May 1;11:535.Read more>>
- YuanYuan Hou. et al. Long-term variable photoperiod exposure impairs the mPFC and induces anxiety and depression-like behavior in male wistar rats. Exp Neurol. 2022 Jan;347:113908Read more>>
- Joanna Winstone. et al. Effects of Tetrahydrocannabinol and Cannabidiol on Brain-Derived Neurotrophic Factor and Tropomyosin Receptor Kinase B Expression in the Adolescent Hippocampus. CANNABIS CANNABINOID. 2022 May 3Read more>>
- Molly Kent. et al. The Emotional Impact of Disrupted Environmental Contexts: Enrichment loss and coping profiles influence stress response recovery in Long-Evans rats. J NEUROENDOCRINOL. 2022 Jun 22Read more>>
- Song Qiaoli. et al. Neuroprotective Effects of Estrogen Through BDNF-Transient Receptor Potential Channels 6 Signaling Pathway in the Hippocampus in a Rat Model of Perimenopausal Depression. FRONT AGING NEUROSCI. 2022 Jul;0:675Read more>>
- Akira Tani. et al. Stimulation of functional recovery via neurorepair mechanisms by the traditional Japanese Kampo medicine, Ninjin'yoeito, and physical exercise in a rat ischemic stroke model. J ETHNOPHARMACOL. 2023 Feb;302:115927Read more>>
- Sheng-Hua Wu. et al. Spinal Irisin Gene Delivery Attenuates Burn Injury-Induced Muscle Atrophy by Promoting Axonal Myelination and Innervation of Neuromuscular Junctions. INT J MOL SCI. 2022 Jan;23(24):15899Read more>>
- Tingting Ku. et al. Tebuconazole mediates cognitive impairment via the microbe-gut-brain axis (MGBA) in mice. ENVIRON INT. 2023 Feb;:107821Read more>>
- Liu Sumei. et al. Suppression of TGFR-Smad3 pathway alleviates the syrinx induced by syringomyelia. CELL BIOSCI. 2023 Dec;13(1):1-13Read more>>
- Kazuki Nakanishi. et al. Effects of early exercise intervention and exercise cessation on neuronal loss and neuroinflammation in a senescence-accelerated mouse prone 8. NEUROSCI LETT. 2023 Jun;808:137297Read more>>
- Yingqian Zhang. et al. Milk fat globule membrane promotes brain development in piglets by enhancing the connection of white matter fiber trace. FRONT NUTR. 2023; 10: 1248809Read more>>
- Cahide Aslan. et al. Protective Effect of Vitamin D on Learning and Memory Impairment in Rats Induced by High Fructose Corn Syrup. BEHAV BRAIN RES. 2023 Nov;:114763Read more>>
- Lan Yan-Ping. et al. Analysis of the functional role and mRNA expression of GABABR in the nucleus accumbens of cocaine-addicted rats. J CHIN MED ASSOC. 2024 Jun;:10.1097/JCMA.0000000000001119Read more>>
- Zhao Yu-Long. et al. Activation of 5-HT6 Receptors in the Ventrolateral Orbital Cortex Produces Anti-Anxiodepressive Effects in a Rat Model of Neuropathic Pain. MOL NEUROBIOL. 2024 Jul;:1-15Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat brain labeled with Anti-BDNF Polyclonal Antibody, Unconjugated (bs-4989R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
U251 cells; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37C for 20 min; Antibody incubation with BDNF Polyclonal Antibody, Unconjugated (bs-4989R) 1:200, 90 minutes at 37C; followed by a conjugated secondary antibody (bs-0295G-FITC) at 37C for 90 minutes, DAPI (5ug/ml) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with BDNF Polyclonal Antibody (bs-4989R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
HepG2 cells were fixed with 4% PFA for 10min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with BDNF Polyclonal Antibody(bs-4989R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), no antibody control control (blue), secondary antibody only (light blue) and isotype control (orange).
Lane 1: Mouse Cerebrum cell lysates probed with BDNF Polyclonal Antibody, Unconjugated (bs-4989R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with BDNF Polyclonal Antibody, Unconjugated (bs-4989R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BDNF) Polyclonal Antibody, Unconjugated (bs-4989R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with BDNF polyclonal antibody, unconjugated (bs-4989R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.