bs-4943R
[Primary Antibody]
MPO Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: MPO
Immunogen Range: 678-745/745
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4353
Swiss Prot: P05164
Source: KLH conjugated synthetic peptide derived from human MPO
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
Size: 100ul
Concentration: 1ug/ul
Applications:
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 84
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Pig
Horse
Rabbit
Guinea Pig
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Hu, Hai Xia, et al. "Anti-inflammatory effects of Gualou Guizhi decoction in transient focal cerebral ischemic brains. Corrigendum in/mmr/12/3/3998." Molecular medicine reports 12.1 (2015): 1321-1327.Read more>>
- Zheng, Jian, et al. "Lithium posttreatment confers neuroprotection through glycogen synthase kinase-3β inhibition in intracerebral hemorrhage rats." Journal of Neurosurgery (2016): 1-9.Read more>>
- Thais Fantozzi,et al.Acute lung injury induced by intestinal ischemia and reperfusion is altered in obese female mice.(2018) Pulmonary Pharmacology & Therapeutics. 49:54-59.Read more>>
- Sekerci,et al.Apelin/APJ expression in the heart and kidneys of hypertensive rats.(2018) Acta Histochemica. 120:196-204.Read more>>
- Li Y et al. Role of intestinal microbiota-mediated genipin dialdehyde intermediate formation in geniposide-induced hepatotoxicity in rats. Toxicol Appl Pharmacol. 2019 Jun 11;377:114624. Read more>>
- Emilia A Barbuet al. Detection and Quantification of Histone H4 Citrullination in Early NETosis With Image Flow Cytometry Version 4. Front Immunol. 2020 Jul 16;11:1335.Read more>>
- oon Ching Teeet al. Xenogeneic mesenchymal stem cell transplantation for mandibular defect regeneration. Xenotransplantation. 2020 Sep;27(5):e12625.Read more>>
- Tae-Young Kim. et al. Facilitation of Bone Healing Processes Based on the Developmental Function of Meox2 in Tooth Loss Lesion. Int J Mol Sci. 2020 Jan;21(22):8701Read more>>
- Kyle Jablonski. et al. Physical activity prevents acute inflammation in a gout model by downregulation of TLR2 on circulating neutrophils as well as inhibition of serum CXCL1 and is associated with decreased pain and inflammation in gout patients. Plos One. 2020 Oct;15(10):e023752Read more>>
- Chuntong Bao. et al. IFN--/- mice resist Actinobacillus pleuropneumoniae infection by promoting early lung IL-18 release and PMN-I accumulation. Infect Immun. 2021 Mar;:IAI.00069-21Read more>>
- Yin Xian. et al. Sleeve Gastrectomy Attenuates the Severity of Cerulein-Induced Acute Pancreatitis in Obese Rats. 2021 Jun 21Read more>>
- Ye, Buqing. et al. Induction of functional neutrophils from mouse fibroblasts by thymidine through enhancement of Tet3 activity. Cell Mol Immunol. 2022 Mar;:1-15Read more>>
- Noive Arteche-Villasol. et al. Assessment of Paratuberculosis Vaccination Effect on In Vitro Formation of Neutrophil Extracellular Traps in a Sheep Model. VACCINES-BASEL. 2022 Sep;10(9):1403Read more>>
- Uygar Sa??k. et al. Effect of ferulic acid on testicular damage caused by torsion-detorsion in rats. BIOTECH HISTOCHEM. 2022 Aug 23Read more>>
- ae Ju-Eun. et al. Effects of erythropoietin on osteoblast in the tooth extraction socket in mice periodontitis model. FRONT PHYSIOL. 2022 Oct;0:2117Read more>>
- Li Yue. et al. Effects of resveratrol and its derivative pterostilbene on hepatic injury and immunological stress of weaned piglets challenged with lipopolysaccharide. J ANIM SCI. 2022 OcRead more>>
- Criado Miguel. et al. Evaluation of the innate immune response of caprine neutrophils against Mycobacterium avium subspecies paratuberculosis in vitro. VET RES. 2023 Dec;54(1):1-16Read more>>
- Hao Gao. et al. Electroacupuncture treatment improves postoperative ileus by inhibiting the Th1 cell-mediated inflammatory response through the vagus nerve. ACUPUNCT MED. ;()Read more>>
- Miguel Criado. et al. No Evidence of Neutrophil Response Modulation in Goats after Immunization against Paratuberculosis with a Heat-Inactivated Vaccine. ANIMALS. 2024 Jan;14(11):1694Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat brain labeled with Anti-MPO Polyclonal Antibody, Unconjugated (bs-4943R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
HL-60 cells were fixed with 4% PFA for 10min at room temperature,20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MPO Polyclonal Antibody (bs-4943R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MPO Polyclonal Antibody(bs-4943R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
HL60 cell; 4% Paraformaldehyde-fixed; Ice-cold methanol at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Anti-MPO) polyclonal antibody, A555 conjugated (bs-4943R-A555) 1:50, 90 minutes at 37°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
HL60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MPO Polyclonal Antibody(bs-4943R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).