Host: Rabbit
Target Protein: Metallothionein 3
Immunogen Range: 1-68/68
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4504
Swiss Prot: P25713
Source: KLH conjugated synthetic peptide derived from human Metallothionein 3
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
Predicted Molecular Weight: 7.5
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-MT3/Metallothionein 3 Polyclonal Antibody, Unconjugated (bs-4940R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
SH-SY5Y cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Metallothionein 3 Antibody(bs-4940R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).