bs-4900R-Cy5 [Conjugated Primary Antibody]
KEAP1 Polyclonal Antibody, Cy5 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: KEAP1

Immunogen Range: 21-120/624


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 9817

Swiss Prot: Q14145

Source: KLH conjugated synthetic peptide derived from human KEAP1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.

Conjugation: Cy5

Excitation/ Emission: 625,650nm/670nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 69


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Pig
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Li YH et al. GanMeijian Ameliorates Lipid Accumulation and Oxidative Damage in Alcoholic Fatty Liver Disease in Wistar Rats. Life Sci. 2020 Apr30;255:117721.Read more>>
  • Kaiqiang Fu. et al. Berberine attenuates the inflammatory response by activating the Keap1/Nrf2 signaling pathway in bovine endometrial epithelial cells. Int Immunopharmacol. 2021 Jul;96:107738Read more>>
  • Quanwei Li. et al. Toxicological mechanism of large amount of copper supplementation: Effects on endoplasmic reticulum stress and mitochondria-mediated apoptosis by Nrf2/HO-1 pathway-induced oxidative stress in the porcine myocardium. J Inorg Biochem. 2022 May;230:11175Read more>>
  • Rao, Gan. et al. Arsenic Trioxide Triggers Mitochondrial Dysfunction, Oxidative Stress, and Apoptosis via Nrf 2/Caspase 3 Signaling Pathway in Heart of Ducks. BIOL TRACE ELEM RES. Biol Trace Elem Res. 2022 Apr;:1-11Read more>>
  • Chen, Bo. et al. Acute Aluminum Sulfate Triggers Inflammation and Oxidative Stress, Inducing Tissue Damage in the Kidney of the Chick. BIOL TRACE ELEM RES. 2022 May;:1-9Read more>>
  • Zhihui Liu. et al. Curcumin alleviates aristolochic acid nephropathy based on SIRT1/Nrf2/HO-1 signaling pathway. TOXICOLOGY. 2022 Sep;479:153297Read more>>
  • Mengru Li. et al. The leaves of Scutellaria baicalensis Georgi attenuate brain aging in D-galactose-induced rats via regulating glutamate metabolism and Nrf2 signaling pathway. EXP GERONTOL. 2022 Dec;170:111978Read more>>
  • Rui Sang. et al. Taraxasterol alleviates aflatoxin B1-induced liver damage in broiler chickens via regulation of oxidative stress, apoptosis and autophagy. ECOTOX ENVIRON SAFE. 2023 Feb;251:114546Read more>>
  • Hong Zhao. et al. Polysaccharides from sea buckthorn (Hippophae rhamnoides L.) berries ameliorates cognitive dysfunction in AD mice induced by a combination of D-gal and AlCl3 by suppressing oxidative stress and inflammation reaction. J SCI FOOD AGR. 2023 MayRead more>>
  • Shanshan Qi. et al. The hepatoprotective effect of Sophora viciifolia fruit extract against acetaminophen-induced liver injury in mice. J APPL BIOMED. 2023 May 18Read more>>
  • Qingyu Ding. et al. N-acetylcysteine alleviates oxidative stress and apoptosis and prevents skeletal muscle atrophy in type 1 diabetes mellitus through the NRF2/HO-1 pathway. LIFE SCI. 2023 Sep;329:121975Read more>>
  • Ming Zhou. et al. Protective effect of ginsenoside Rg1 on 661W cells exposed to oxygen-glucose deprivation/reperfusion via keap1/nrf2 pathway. INT J OPHTHALMOL-CHI. 2023; 16(7): 1026C1033Read more>>
  • Yaqi Li. et al. Non-thermal plasma promotes boar sperm quality through increasing AMPK methylation. INT J BIOL MACROMOL. 2024 Feb;257:128768Read more>>
  • Xuanxuan Jiang. et al. Multi-omics reveals the protective effects of curcumin against AFB1-induced oxidative stress and inflammatory damage in duckling intestines. COMP BIOCHEM PHYS C. 2024 Feb;276:109815Read more>>
  • Jixian Song. et al. Hydrogen Attenuates Chronic Intermittent Hypoxia-Induced Cardiac Hypertrophy by Regulating Iron Metabolism. CURR ISSUES MOL BIOL. 2023 Dec;45(12):10193-1021Read more>>
  • Lei Liu. et al. Myricetin Oligomer Triggers Multi-Receptor Mediated Penetration and Autophagic Restoration of Blood-Brain Barrier for Ischemic Stroke Treatment. ACS NANO. 2024;XXXX(XXX):XXX-XXXRead more>>
  • Mingran Dong. et al. Anthocyanins from Lycium ruthenicum Murray Mitigate Cadmium-Induced Oxidative Stress and Testicular Toxicity by Activating the Keap1/Nrf2 Signaling Pathway. PHARMACEUTICALS-BASE. 2024 Mar;17(3):322Read more>>
  • Hongying Dong. et al. Pueraria lobata antioxidant extract ameliorates non-alcoholic fatty liver by altering hepatic fat accumulation and oxidative stress. J ETHNOPHARMACOL. 2024 Oct;333:118468Read more>>
  • Gege Wei. et al. Identification and functional prediction of miRNAs that regulate ROS levels in dielectric barrier discharge plasma-treated boar spermatozoa. THERIOGENOLOGY. 2024 JuRead more>>
  • Sneha Kumari. et al. Understanding the molecular basis of anti-fibrotic potential of intranasal curcumin and its association with mitochondrial homeostasis in silica-exposed mice. MITOCHONDRION. 2024 Sep;78:101943Read more>>
  • Xi-Kun Yuan. et al. Effects of Life-Long Exercise on Age-Related Inflammation, Apoptosis, Oxidative Stress, Ferroptosis Markers, and NRF2/KAEP 1/Klotho Pathway in Rat Kidneys. PHYSIOL RES. 2024 Aug 31;73(4):577-591Read more>>
VALIDATION IMAGES