bs-4843R
[Primary Antibody]
LC3B Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: LC3B
Clonality: Polyclonal
Isotype: IgG
Source: KLH conjugated synthetic peptide derived from human Microtubule-associated proteins 1A/1B light chain 3B
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and in maintaining the balance between neuronal plasticity and rigidity. MAP-light chain 3 beta (MAP-LC3 Beta) and MAP-light chain 3 alpha (MAP-LC3 Alpha) are subunits of both MAP1A and MAP1B. MAP-LC3 Beta, a homolog of Apg8p, is essential for autophagy and associated to the autophagosome membranes after processing. Two forms of LC3 Beta, the cytosolic LC3-I and the membrane-bound LC3-II, are produced post-translationally. LC3-I is formed by the removal of the C-terminal 22 amino acids from newly synthesized LC3 Beta, followed by the conversion of a fraction of LC3-I into LC3-II. LC3 enhances fibronectin mRNA translation in ductus arteriosus cells through association with 60S ribosomes and binding to an AU-rich element in the 3’ untranslated region of fibronectin mRNA. This facilitates sorting of fibronectin mRNA onto rough endoplasmic reticulum and translation. MAP LC3 Beta may also be involved in formation of autophagosomal vacuoles. It is expressed primarily in heart, testis, brain and skeletal muscle.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
FCM(1:20-100)
IHC-P(1:200-400)
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Li et al. Apolipoprotein E-Mimetic Peptide COG1410 Promotes Autophagy by Phosphorylating GSK-3β in Early Brain Injury Following Experimental Subarachnoid Hemorrhage. (2018) Front.Neurosci. 12:127Read more>>
- Wang et al. Expression and clinical significance of autophagic protein LC3B and EMT markers in gastric cancer. (2018) Cancer.Manag.Res. 10:1479-1486Read more>>
- Qingyu Meng. et al. Morin hydrate inhibits atherosclerosis and LPS-induced endothelial cells inflammatory responses by modulating the NFB signaling-mediated autophagy. Int Immunopharmacol. 2021 Nov;100:108096Read more>>
- Ziyu Meng. et al. Sesamin promotes apoptosis and pyroptosis via autophagy to enhance antitumour effects on murine T-cell lymphoma. J Pharmacol Sci. 2021 Nov;147:26Read more>>
- Liwen Luo. et al. Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration. Bioact Mater. 2021 DecRead more>>
- Chenliang Zhang. et al. PKM2 compensates for proteasome dysfunction by mediating the formation of the CHIP-HSP70-BAG3 complex?and the aggregation of ubiquitinated proteins. Faseb J. 2022 Jan;36(1):e22121Read more>>
- Pan Huang. et al. Long-term treatment of Nicotinamide mononucleotide improved Age-related Diminished Ovary Reserve through enhancing the mitophagy level of granuloas cellsin mice. J Nutr Biochem. 2021 Nov;:108911Read more>>
- Wang Zhiwen. et al. Cinnamaldehyde Downregulation of Sept9 Inhibits Glioma Progression through Suppressing Hif-1_ via the Pi3k/Akt Signaling Pathway. Dis Markers. 2022;2022:6530934Read more>>
- Shu-wen Xu. et al. Cigarette smoke extract-induced inflammatory response via inhibition of the TFEB-mediated autophagy in NR8383 cells. EXP LUNG RES. 2023 Jan 13Read more>>
- Zhengda Li. et al. METTL3-mediated m6A methylation regulates granulosa cells autophagy during follicular atresia in pig ovaries. THERIOGENOLOGY. 2023 Apr;201:83Read more>>
- Xiangyu Wang. et al. CERS6-derived ceramides aggravate kidney fibrosis by inhibiting PINK1-mediated mitophagy in diabetic kidney disease. AM J PHYSIOL-CELL PH. 2023 Jul;(Articles in Press)Read more>>
- Zhanglu Fang. et al. Effects of Neuroinflammation and Autophagy on the Structure of the BloodCBrain Barrier in ADHD Model. NEUROSCIENCE. 2023 AugRead more>>
- Huijuan Zhang. et al. In situ autophagy regulation in synergy with phototherapy for breast cancer treatment. ACTA PHARM SIN B. 2023 NovRead more>>
- Haolin Duan. et al. MT-TN mutations lead to progressive mitochondrial encephalopathy and promotes mitophagy. BBA-MOL BASIS DIS. 2024 Apr;1870:167043Read more>>
- Zetao Wang. et al. Nano-vibration exciter: Hypoxia-inducible factor 1 signaling pathway-mediated extracellular vesicles as bioactive glass substitutes for bone regeneration. BIOACT MATER. 2024 Oct;40:46Read more>>
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with LC3B Polyclonal Antibody, Unconjugated (bs-4843R) at 1:200 overnight at 4°C, DAB staining.
Lane 1:Rat Cerebrum lysates; Lane 2: Human SH-SY5Y cell lysates; Lane 3: Human Hela cell lysates; Lane 4: Human HepG2 cell lysates probed with LC3B Polyclonal Antibody, Unconjugated (bs-4843R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Mouse Testis tissue lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Rat Cerebellum tissue lysates; Lane 6: Rat Testis tissue lysates probed with LC3B Polyclonal Antibody, Unconjugated (bs-4843R) at dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.