bs-4842R-Biotin [Conjugated Primary Antibody]
eIF2 alpha subunit 1 (Ser52) Antibody, Biotin Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: eIF2 alpha subunit 1 Ser52

Modification Site: Ser52

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1965

Swiss Prot: P05198

Source: KLH conjugated synthetic phosphopeptide derived from human eIF2 alpha subunit 1 around the phosphorylation site of Ser52 [EL(p-S)RR]

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 4S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 6S ribosomal subunit to form the 8S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Conjugation: Biotin

Excitation/ Emission: N/A

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)

Predicted Molecular Weight: 36


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • He, Yihuai, et al. "Sustained endoplasmic reticulum stress inhibits hepatocyte proliferation via downregulation of c-Met expression." Molecular and Cellular Biochemistry (2014): 1-8.Read more>>
  • Yao et al. A non-canonical pathway regulates ER stress signaling and blocks ER stress-induced apoptosis and heart failure. (2017) Nat.Commu. 8:133Read more>>
  • Li,et al.Silver nanoparticles induce SH-SY5Y cell apoptosis via endoplasmic reticulum- and mitochondrial pathways that lengthen endoplasmic reticulum-mitochondria contact sites and alter inositol-3-phosphate receptor function.(2018) Toxicology Letters. 285:156-167.Read more>>
  • Berger,et al.Localized Myosin II Activity Regulates Assembly and Plasticity of the Axon Initial Segment.(2018) Neuron. 97:555-570.e6.Read more>>
  • Du,et al.A Novel and Convenient Method for the Preparation and Activation of PRP without Any Additives: Temperature Controlled PRP.(2018) BioMed Research International. 2018:1761865.Read more>>
  • Cha et al. miR-211 Plays a Critical Role in Cnidium officinale Makino Extract-Induced, ROS/ER Stress-Mediated Apoptosis in U937 and U266 Cells. (2018) Int.J.Mol.Sci.Mar 15;19(3). Read more>>
  • ingwei Yang et al. Polychlorinated Biphenyl Quinone Promotes Atherosclerosis through Lipid Accumulation and Endoplasmic Reticulum Stress via CD36. Chem Res Toxicol. 2020 Jun 15;33(6):1497-1507.Read more>>
  • Wenfeng Gouet al. Ursolic acid derivative UA232 evokes apoptosis of lung cancer cells induced by endoplasmic reticulum stress. Pharm Biol. 2020 Dec;58(1):707-715.Read more>>
  • Qiyi Zhao. et al. Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output. Cell. 2020 Oct;183:76Read more>>
  • Yu Wang. et al. Zinc offers splenic protection through suppressing PERK/IRE1-driven apoptosis pathway in common carp (Cyprinus carpio) under arsenic stress. Ecotox Environ Safe. 2021 Jan;208:111473Read more>>
  • Tingting Wang. et al. Synergistic Lysosomal Impairment and ER Stress Activation for Boosted Autophagy Dysfunction Based on Te Double-Headed Nano-Bullets. SMALL. 2022 May 29Read more>>
  • Yue Zhang. et al. Endoplasmic reticulum stress-controlled autophagic pathway promotes polystyrene microplastics-induced myocardial dysplasia in birds. ENVIRON POLLUT. 2022 Oct;311:119963Read more>>
  • Chen Yanan. et al. Pterostilbene attenuates intrauterine growth retardation-induced colon inflammation in piglets by modulating endoplasmic reticulum stress and autophagy. Journal of Animal Science and Biotechnology. 2022 Dec;13(1):1-16Read more>>
  • Tao Liu. et al. Dapagliflozin attenuates cardiac remodeling and dysfunction in rats with -adrenergic receptor overactivation through restoring calcium handling and suppressing cardiomyocyte apoptosis. DIABETES VASC DIS RE. ;()Read more>>
  • Zhou Yanyan. et al. Homoplantaginin attenuates high glucose-induced vascular endothelial dysfunction via inhibiting store-operated calcium entry channel and endoplasmic reticulum stress. J PHARM PHARMACOL. 2023 SepRead more>>
VALIDATION IMAGES