bs-4826R [Primary Antibody]
CD83 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD83

Immunogen Range: 32-100/205


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 9308

Swiss Prot: Q01151

Source: KLH conjugated synthetic peptide derived from human CD83

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)

Predicted Molecular Weight: 21


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Mouse splenocytes probed with Rabbit Anti-CD83 Polyclonal Antibody, PE Conjugated (bs-4826R) (green) at 1:20 for 40 minutes compared to control cells (blue).


A431 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD83 Antibody(bs-4826R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).