bs-4821R [Primary Antibody]
AFP Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: AFP

Immunogen Range: 501-609/609


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 174

Swiss Prot: P02771

Source: KLH conjugated synthetic peptide derived from human Alpha Fetoprotein

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 65


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Mo G et al.A novel ECL sensor based on a boronate affinity molecular imprinting technique and functionalized SiO@CQDs/AuNPs/MPBA nanocomposites for sensitive determination of alpha-fetoprotein.(2019)Biosens Bioelectron. Feb 1;126:558-564.Read more>>
  • Chen YJ et al. A TiO2 mesocrystals triggered signal amplification strategy of photoelectrochemical immunoassay for α-fetoprotein detection based on indirect competitive reaction. Journal of Electroanalytical Chemistry. 2019 July 16.113313.Read more>>
  • Zhou X et al. A Gold Nanoparticle Probe-Assisted Antigen-Counting Chip using SEM. ACS Appl Mater Interfaces. 2019 Feb 20;11(7):6769-6776. Read more>>
  • Chellachamy Anbalagan, Amarnath. et al. Carboxylic acidCtethered polyaniline as a generic immobilization matrix for electrochemical bioassays. Microchim Acta. 2021 Nov;188(11):1-12Read more>>
  • Chellachamy Anbalagan, Amarnath. et al. Carboxylic acidCtethered polyaniline as a generic immobilization matrix for electrochemical bioassays. Microchim Acta. 2021 Nov;188(11):1-12Read more>>
VALIDATION IMAGES

HepG2 lysates probed with AFP Polyclonal Antibody, Unconjugated (bs-4821R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin-embedded Human Liver Cancer tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with AFP Polyclonal Antibody, Unconjugated (bs-4821R) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody, DAB and counterstaining.


25 ug total protein per lane of various lysates (see on figure) probed with AFP polyclonal antibody, unconjugated (bs-4821R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.