DATASHEET
Host:
Rabbit
Target Protein:
CD3E
Immunogen Range:
23-120/189
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
12501
Swiss Prot:
P22646
Source:
KLH conjugated synthetic peptide derived from mouse CD3 epsilon
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
The CD3 complex mediates signal transduction.
PRODUCT SPECIFIC PUBLICATIONS
- Chenna Narendra S, Chalise JP, Magnusson M, Uppugunduri S (2015) Local but Not Systemic Administration of Uridine Prevents Development of Antigen-Induced Arthritis. PLoS ONE 10(10): e0141863Read more>>
- Da?Won Choi. et al. Co?transplantation of tonsil?derived mesenchymal stromal cells in bone marrow transplantation promotes thymus regeneration and T cell diversity following cytotoxic conditioning. Int J Mol Med. 2020 Sep;46(3):1166-1174Read more>>
- Quanyu Chen. et al. Comparative effects of hepatocyte growth factor and tacrolimus on acute liver allograft early tolerance. FRONT IMMUNOL. 2023; 14: 1162439Read more>>
VALIDATION IMAGES
Mouse spleen lysate probed with Anti-CD3E Polyclonal Antibody, Unconjugated (bs-4815R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
Lane 1: Human MOLT4 cell lysates; Lane 2: Human Jurkat cell lysates probed with CD3E Polyclonal Antibody, Unconjugated (bs-4815R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.