bs-4770R
[Primary Antibody]
CD133 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: CD133
Immunogen Range: 508-552/865
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 8842
Swiss Prot: O43490
Source: KLH conjugated synthetic peptide derived from human CD133
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439).
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
Predicted Molecular Weight: 95
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species: Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Qi et al. Targeting the Wnt-Regulatory Protein CTNNBIP1 by microRNA-214 Enhances the Stemness and Self-Renewal of Cancer Stem-Like Cells in Lung Adenocarcinomas. (2015) Stem.Cells. 33(12):3423-36Read more>>
- Sun Z et al. Glioblastoma Stem Cell-Derived Exosomes Enhance Stemness and Tumorigenicity of Glioma Cells by Transferring Notch1 Protein. Cell Mol Neurobiol. 2019 Dec 18. Read more>>
- Dworacka M et al. Simvastatin attenuates the aberrant expression of angiogenic factors induced by glucose variability.Diabetes Res Clin Pract. 2018 Sep;143:245-253.Read more>>
- Wang L et al. Lung CSC\derived exosomal miR\210\3p contributes to a pro\metastatic phenotype in lung cancer by targeting FGFRL1. J Cell Mol Med. 2020 Jun;24(11):6324-6339.Read more>>
- Wei Wang. et al. The effect of endothelial progenitor cell transplantation on neointimal hyperplasia and reendothelialisation after balloon catheter injury in rat carotid arteries. Stem Cell Res Ther. 2021 Dec;12(1):1-12Read more>>
- Luo Dan. et al. Premobilization of CD133+ progenitors is associated with attenuated inflammation-induced pulmonary dysfunction following extracorporeal circulation in mice. Interact Cardiov Th. 2020 Aug;31(2):210-22Read more>>
- Jingyu Liu. et al. Low\energy shock wave pretreatment recruit circulating endothelial progenitor cells to attenuate renal ischaemia reperfusion injury. J Cell Mol Med. 2020 Sep;24(18):10589-10603Read more>>
- Yang Yang. et al. PT109, a novel multi-kinase inhibitor suppresses glioblastoma multiforme through cell reprogramming: Involvement of PTBP1/PKM1/2 pathway. Eur J Pharmacol. 2022 Apr;920:174837Read more>>
- Wu Cuiling. et al. Regulation of secondary hair follicle cycle in cashmere goats by miR-877-3p targeting IGFBP5 gene. J ANIM SCI. 2023 SepRead more>>
- Ruiqian Sun. et al. Astragali Radix-Curcumae Rhizoma herb pair reduces the stemness of colorectal cancer cells through HIF-2/-catenin pathway. PHYTOMEDICINE. 2024 Sep;132:155824Read more>>
VALIDATION IMAGES
LoVo lysates probed with CD133 Polyclonal Antibody, Unconjugated (bs-4770R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD133 Polyclonal Antibody, Unconjugated (bs-4770R) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD133 Polyclonal Antibody, Unconjugated (bs-4770R) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Lane 1: Mouse Sp2/0 cell lysates; Lane 2: Human LOVO cell lysates; Lane 3: Human SW480 cell lysates; Lane 4: Mouse Uterus lysates probed with CD133 Polyclonal Antibody, Unconjugated (bs-4770R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Mouse kidney cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD133 Antibody(bs-4770R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Sp2/0 cell lysates; Lane 2: Human LOVO cell lysates; Lane 3: Human SW480 cell lysates; Lane 4: Mouse Uterus cell lysates probed with CD133 Polyclonal Antibody, Unconjugated (bs-4770R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.