bs-4754R [Primary Antibody]
VWF Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: VWF

Immunogen Range: 351-450/2813


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7450

Swiss Prot: P04275

Source: KLH conjugated synthetic peptide derived from human VWF/von Willebrand antigen 2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.

Size: 100ul

Concentration: 1ug/ul

Applications: FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 81


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xenia Kraus. et al. A pre-conditioning protocol of peripheral blood derived endothelial colony forming cells for endothelialization of tissue engineered constructs. Microvasc Res. 2021 Mar;134:104107Read more>>
  • Fei Sun. et al. Biomimetic in situ tracheal microvascularization for segmental tracheal reconstruction in one-step. BIOENG TRANSL MED. 2023 May;:e10534Read more>>
  • Shi Xin. et al. The bone marrow endothelial progenitor cell response to septic infection. FRONT IMMUNOL. 2024 Apr;15Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with VWF Polyclonal Antibody (bs-4754R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.


Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with VWF Polyclonal Antibody (bs-4754R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.