Host: Rabbit
Target Protein: CD168
Immunogen Range: 51-150/725
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3161
Swiss Prot: O75330
Source: KLH conjugated synthetic peptide derived from human CD168
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 84
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species:
Rat
Cow
Horse
For research use only. Not intended for diagnostic or therapeutic use.
- Hagey DW et al. CYCLIN-B1/2 and -D1 act in opposition to coordinate cortical progenitor self-renewal and lineage commitment. Nat Commun. 2020 Jun 9;11(1):2898.Read more>>
Mouse bone lysate 30ug, probed with Anti-CD168 Polyclonal Antibody (bs-4736R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.
U-937 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD168 Polyclonal Antibody(bs-4736R-AF488)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).