bs-4727R
[Primary Antibody]
MRC1 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: MRC1
Immunogen Range: 201-300/1456
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4360
Swiss Prot: P22897
Source: KLH conjugated synthetic peptide derived from human MRC1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
Predicted Molecular Weight: 160
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Karmouty-Quintana, Harry, et al. "Deletion of ADORA2B from myeloid cells dampens lung fibrosis and pulmonary hypertension." The FASEB Journal (2014): fj-14.Read more>>
- Das, Anusuya, et al. "Bioactive lipid coating of bone allografts directs engraftment and fate determination of bone marrow-derived cells in rat GFP chimeras." Biomaterials (2015).Read more>>
- Azad, Abul K., et al. "γ-Tilmanocept, a New Radiopharmaceutical Tracer for Cancer Sentinel Lymph Nodes, Binds to the Mannose Receptor (CD206)." The Journal of Immunology (2015): 1402005.Read more>>
- Locatelli, Luigi, et al. "Macrophage recruitment by fibrocystin‐defective biliary epithelial cells promotes portal fibrosis in congenital hepatic fibrosis."Hepatology (2016).Read more>>
- Struecker, B., et al. "Implantation of a Tissue-Engineered Neo-Bile Duct in Domestic Pigs." European Surgical Research 56.1-2 (2015): 61-75.Read more>>
- Zhu, Dan, et al. "M2 Macrophage Transplantation Ameliorates Cognitive Dysfunction in Amyloid-β-Treated Rats Through Regulation of Microglial Polarization." Journal of Alzheimers Disease: 1-13.Read more>>
- Blomgran, Parmis, et al. "A possible link between loading, inflammation and healing: Immune cell populations during tendon healing in the rat." Scientific Reports 6 (2016): 29824.Read more>>
- Nakai et al. IL-17A induces heterogeneous macrophages, and it does not alter the effects of lipopolysaccharides on macrophage activation in the skin of mice. (2017) Sci.Rep. 7:12473Read more>>
- Nishikawa,et al.Highly Dispersible and Bioavailable Curcumin but not Native Curcumin Induces Brown-Like Adipocyte Formation in Mice.(2018) Molecular Nutrition & Food Research. 62:.Read more>>
- White,et al.GLUT12 promotes prostate cancer cell growth and is regulated by androgens and CaMKK2 signaling.(2018) Endocrine-Related Cancer. 25:453-469.Read more>>
- Shi F et al. Heat injured stromal cells-derived exosomal EGFR enhances prostatic wound healing afterthulium laser resection through EMT and NF-κB signaling. Prostate. 2019 May 24. Read more>>
- Ma R et al. Exploring the interactions between engineered nanomaterials and immune cells at 3D Nano-bio interfaces to discover potent Nano-adjuvants. Nanomedicine. 2019 Jun 18;21:102037. Read more>>
- Xie W et al. Dose Dependent Modulation of Bioactive Glasses Particles on Macrophage and Diabetic Wound Healing. Journal of Materials Chemistry B. 2018.Read more>>
- Žaloudíková M et al. The effect of exposure to hypoxia on superoxide formation by alveolar macrophages is indirect. Life Sci. 2019 Sep 10;236:116864.Read more>>
- Nishikawa S et al. Co-Administration of Curcumin and Artepillin C Induces Development of Brown-Like Adipocytes in Association with Local Norepinephrine Production by Alternatively Activated Macrophages in Mice. J Nutr Sci Vitaminol (Tokyo). 2019;65(4):328-334. Read more>>
- Shao N et al. A Multi‐Functional Silicon Nanoparticle Designed for Enhanced Osteoblast Calcification and Related Combination Therapy. Macromol Biosci. 2019 Nov 10:e1900255. Read more>>
- Jiang L et al. Sonodynamic Therapy in Atherosclerosis by Curcumin Nanosuspensions: Preparation design, Efficacy Evaluation, and Mechanisms Analysis. Eur J Pharm Biopharm. 2020 Jan;146:101-110.Read more>>
- Zhan Q et al. Structural characterization and immunomodulatory activity of a novel acid polysaccharide isolated from the pulp of Rosa laevigata Michx fruit. Int J Biol Macromol. 2019 Nov 12. pii: S0141-8130(19)37108-9.Read more>>
- Liu J et al. Polychlorinated biphenyl quinone promotes macrophage polarization to CD163+ cells through Nrf2 signaling pathway. Environmental Pollution,2019, 113587. Read more>>
- Shao N et al. A Multi‐Functional Silicon Nanoparticle Designed for Enhanced Osteoblast Calcification and Related Combination Therapy. Macromol Biosci. 2019 Nov 10:e1900255. Read more>>
- Liu S et al. Absorbable Thioether Grafted Hyaluronic Acid Nanofibrous Hydrogel for Synergistic Modulation of Inflammation Microenvironment to Accelerate Chronic Diabetic Wound Healing. Adv Healthc Mater. 2020 Apr 27:e2000198.Read more>>
- Tong?L et al. ACT001 reduces the expression of PD-L1 by inhibiting the phosphorylation of STAT3 in glioblastoma. Theranostics. 2020 May 1;10(13):5943-5956.Read more>>
- Gitto SB et al. Identification of a novel IL-5 signaling pathway in chronic pancreatitis and crosstalk with pancreatic tumor cells. Cell Commun Signal. 2020 Jun 17;18(1):95.Read more>>
- Yitian Du. et al. Engineered Microglia Potentiate the Action of Drugs against Glioma Through Extracellular Vesicles and Tunneling Nanotubes. 2021 Feb 28Read more>>
- Hao Liu. et al. A 14 bp indel polymorphism in the promoter region is associated with different responses to porcine circovirus type 2 infection by regulating MRC1 transcription. Vet Immunol Immunop. 2021 Apr;234:110202Read more>>
- Sha Liu. et al. Dual Cross-linked HHA Hydrogel Supplies and Regulates M2 for Synergistic Improvement of Immunocompromise and Impaired Angiogenesis to Enhance Diabetic Chronic Wound Healing. Biomacromolecules. 2020;21(9):3795C3806Read more>>
- Jiyuan Piao. et al. Substance P blocks -aminopropionitrile-induced aortic injury through modulation of M2 monocyte-skewed monocytopoiesis. Transl Res. 2021 Feb;228:76Read more>>
- Xiaowei Li. et al. Pristane promotes anaerobic glycolysis to facilitate proinflammatory activation of macrophages and development of arthritis. Exp Cell Res. 2021 Jan;398:112404Read more>>
- Simon Harper. et al. Portal venous repopulation of decellularised rat liver scaffolds with syngeneic bone marrow stem cells. J Tissue Eng Regen M. 2020 Oct;14(10):1502-1512Read more>>
- Kohei Kamada. et al. Attenuation of Knee Osteoarthritis Progression in Mice through Polarization of M2 Macrophages by Intra-Articular Transplantation of Non-Cultured Human Adipose-Derived Regenerative Cells. J Clin Med. 2021 Jan;10(19):4309Read more>>
- Meng Lin. et al. CRISPR-based in situ engineering tumor cells to reprogram macrophages for effective cancer immunotherapy. Nano Today. 2022 Feb;42:101359Read more>>
- Nguyen, Thao K. T.. et al. Recombinant Human Lactoferrin Reduces Inflammation and Increases Fluoroquinolone Penetration to Primary Granulomas During Mycobacterial Infection of C57Bl/6 Mice. Arch Immunol Ther Ex. 2022 Dec;70(1):1-13Read more>>
- Jing Wang. et al. Mesenchymal stem cells regulate M1 polarization of peritoneal macrophages through the CARD9-NF-_B signaling pathway in severe acute pancreatitis. J HEPATO-BIL-PAN SCI. 2022 Jun 23Read more>>
- Xuan Li. et al. ROS-responsive hydrogel coating modified titanium promotes vascularization and osteointegration of bone defects by orchestrating immunomodulation. BIOMATERIALS. 2022 Aug;287:121683Read more>>
- Linghao Xiao. et al. Osteogenic capacity of octacalcium phosphate involving macrophage polarization. J BIOMED MATER RES A. 2022 DecRead more>>
- Xin Li. et al. Paeonol alleviates neuropathic pain by modulating microglial M1 and M2 polarization via the RhoA/p38MAPK signaling pathway. CNS NEUROSCI THER. 2023 ApRead more>>
- Kagioka Takumi. et al. Lithium carbonate accelerates the healing of apical periodontitis. SCI REP-UK. 2023 May;13(1):1-12Read more>>
- Wu Guanghao. et al. Thylakoid engineered M2 macrophage for sonodynamic effect promoted cell therapy of early atherosclerosis. NANO RES. 2023 Sep;:1-1Read more>>
- Yanan Jiang. et al. A MetalCOrganic Framework-Incorporated Hydrogel for Delivery of Immunomodulatory Neobavaisoflavone to Promote Cartilage Regeneration in Osteoarthritis. ACS APPL MATER INTER. 2023;XXXX(XXX):XXX-XXXRead more>>
- Tohru Mutoh. et al. Spatiotemporal expression patterns of ZBP1 in the brain of mouse experimental stroke model. J CHEM NEUROANAT. 2023 Dec;134:102362Read more>>
- Seung-Wan Hong. et al. The Effect of Pregabalin on Microglia Differentiation in Rat with Neuropathic pain: A Preliminary Study. INT J MED SCI. 2024; 21(7): 12651273Read more>>
- Zhuang Cuicui. et al. Lycopene promoted M2 macrophage polarization via inhibition of NOTCH1-PI3K-mTOR-NF-B-JMJD3-IRF4 pathway in response to Escherichia coli infection in J744A.1 cells. ARCH MICROBIOL. 2024 Jun;206(6):1-11Read more>>
- Rebecca A. Stairley. et al. Anti-Ferroptotic Treatment Deteriorates Myocardial Infarction by Inhibiting Angiogenesis and Altering Immune Response. ANTIOXIDANTS-BASEL. 2024 Jul;13(7):769Read more>>
- Vendrov Aleksandr E.. et al. Mitochondrial dysfunction and metabolic reprogramming induce macrophage pro-inflammatory phenotype switch and atherosclerosis progression in aging. FRONT IMMUNOL. 2024 Jun;15Read more>>
- Yan Sisi. et al. CTRP6 alleviates endometrial fibrosis by regulating Smad3 pathway in intrauterine adhesion. BIOL REPROD. 2024 JulRead more>>
VALIDATION IMAGES
HepG2 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MRC1 Antibody（bs-4727R） at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MRC1 Polyclonal Antibody(bs-4727R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
The Molt-4(H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-MRC1 antibody (bs-4727R): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-60295G-FITC): 1 μg/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.