bs-4708R-BF647 [Conjugated Primary Antibody]
AFP Polyclonal Antibody, AbBy Fluor® 647 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: AFP

Immunogen Range: 521-609/609


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 174

Swiss Prot: P02771

Source: KLH conjugated synthetic peptide derived from human AFP

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries.

Conjugation: AbBy Fluor® 647

Excitation/ Emission: 650nm/665nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 65


Cross Reactive Species: Human

Predicted Cross Reactive Species: Monkey

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Li Y et al. Metal Coordination Polymer Induced Perylene Probe Excimer Fluorescence and Its Application in Acetylcholinesterase Sensing and Alpha-Fetoprotein Immunoassay. Analyst. 2019 Mar 21;144(6):2034-2041.Read more>>
  • T H et al. Layer-by-layer self-assembly of MoS2/PDDA hybrid film in microfluidic chips for ultrasensitive electrochemical immunosensing of alpha-fetoprotein. Microchemical Journal,(2020) 158, 105209.Read more>>
  • Guangxian Leng. et al. Alpha-fetoprotein upregulates hepatocellular carcinoma cell-intrinsic PD-1 expression through the LATS2/YAP/TEAD1 pathway. BBA-GEN SUBJECTS. 2024 May;1868:130592Read more>>
VALIDATION IMAGES