bs-4692R [Primary Antibody]
GSK3 Alpha(Ser21) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GSK3 Alpha Ser21

Specificity: This phosphorylation site is homologous to that of Ser21 in Mouse and Rat.

Modification Site: Ser21

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2931

Swiss Prot: P49840

Source: KLH conjugated synthetic phosphopeptide derived from human GSK3 Alpha around the phosphorylation site of Ser21

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1. Requires primed phosphorylation of the majority of its substrates. Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Regulates glycogen metabolism in liver, but not in muscle. May also mediate the development of insulin resistance by regulating activation of transcription factors. In Wnt signaling, regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin. Facilitates amyloid precursor protein (APP) processing and the generation of APP-derived amyloid plaques found in Alzheimer disease. May be involved in the regulation of replication in pancreatic beta-cells. Is necessary for the establishment of neuronal polarity and axon outgrowth. Through phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factors deprivation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 54


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Pig
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhang, Weidong, et al. "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." Chemosphere (2017).Read more>>
VALIDATION IMAGES

Lane 1: rat heart lysates Lane 2: rat brain lysates probed with Anti phospho-GSK3 Alpha(Ser21) Polyclonal Antibody, Unconjugated (bs-4692R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 54kD. Observed band size: 54kD.


Formalin-fixed and paraffin embedded rat brain labeled with Anti-phospho-GSK3 Alpha(Ser21) Polyclonal Antibody, Unconjugated (bs-4692R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: Mouse Thymus tissue lysates; Lane 2: Mouse Liver tissue lysates; Lane 3: Mouse NIH/3T3 cell lysates; Lane 4: Mouse Cerebrum tissue lysates probed with phospho-GSK3A (Ser21) Polyclonal Antibody, Unconjugated (bs-4692R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.