bs-4682R [Primary Antibody]
CD161c/NK1.1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD161c/NK1.1

Immunogen Range: 101-200/223


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 17059

Swiss Prot: P27814

Source: KLH conjugated synthetic peptide derived from mouse CD161/NK1.1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Plays a stimulatory role on natural killer (NK) cells cytotoxicity. Activation by cross-linking of the receptor induces Ca(2+) mobilization and interferon-gamma production.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 25


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Liu et al. B-cell leukemia/lymphoma 10 promotes angiogenesis in an experimental corneal neovascularization model. (2018) Eye.(Lond). Read more>>
  • Zhang Y et al. Recombinant adenovirus expressing a soluble fusion protein PD-1/CD137L subverts the suppression of CD8+ T cells in hepatocellular carcinoma. Mol Ther. 2019 Aug 5. pii: S1525-0016(19)30358-2.Read more>>
  • Uchida T et al. Activated natural killer T cells in mice induce acute kidney injury with hematuria through possibly common mechanisms shared by human CD56+ T cells.Am J Physiol Renal Physiol. 2018 Sep 1;315(3):F618-F627. Read more>>
  • Uchida T et al. Activated natural killer T cells in mice induce acute kidney injury with hematuria through possibly common mechanisms shared by human CD56+ T cells.Am J Physiol Renal Physiol. 2018 Sep 1;315(3):F618-F627. Read more>>
  • Ina B?hr. et al. Characterization of natural killer cells in colorectal tumor tissue of rats fed a control diet or a high-fat diet. Ann Anat. 2021 Jan;233:151586Read more>>
  • Kim, Hyun-Jin. et al. Blood monocyte-derived CD169+ macrophages contribute to antitumor immunity against glioblastoma. NAT COMMUN. 2022 Oct;13(1):1-14Read more>>
  • Leimbacher Aurelia C.. et al. Voluntary Exercise Does Not Always Suppress Lung Cancer Progression. ISCIENCE. 2023 Jan 31Read more>>
  • Miguel A. Moreno-Gonzalez. et al. Combination of cowpea mosaic virus (CPMV) intratumoral therapy and oxaliplatin chemotherapy. Materials Advances. 2024 MayRead more>>
VALIDATION IMAGES

Lane 1: mouse intestine lysates Lane 2: mouse liver lysates probed with Anti CD161c/NK1.1 Polyclonal Antibody, Unconjugated (bs-4682R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 25kD. Observed band size: 25kD.


Mouse plasma probed with Rabbit Anti-CD161c Polyclonal Antibody, Unconjugated (bs-4682R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.


Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD161c/NK1.1 Polyclonal Antibody, Unconjugated (bs-4682R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.


Lane 1: Human U937 cell Lysates; Lane 2: Human Jurkat cell Lysates; Lane 3: Human MOLT4 cell Lysates; Lane 4: Human MCF-7 cell Lysates. Probed with CD161c polyclonal Antibody, unconjugated (bs-4682R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.