bs-4630R
[Primary Antibody]
ROCK1(Thr455/Ser456) Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: ROCK1 Thr455 + Ser456
Modification Site: Thr455/Ser456
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 6093
Swiss Prot: Q13464
Source: KLH conjugated synthetic phosphopeptide derived from human ROCK1 around the phosphorylation site of Thr455/Ser456
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 158
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Wang, Nan, et al. "Vascular endothelial growth factor stimulates endothelial differentiation from mesenchymal stem cells via Rho/myocardin-related transcription factor-A signaling pathway." The International Journal of Biochemistry & Cell Biology (2013).Read more>>
- Lin, Ping-Yuan, et al. "RhoA/ROCK1 regulates Avian Reovirus S1133-induced switch from autophagy to apoptosis." BMC veterinary research 11.1 (2015): 103.Read more>>
- Choi et al. GM-CSF reduces expression of chondroitin sulfate proteoglycan (CSPG) core proteins in TGF-β-treated primary astrocytes. (2014) BMB.Re. 47:679-84Read more>>
- Kim et al. Up-regulation of neogenin-1 increases cell proliferation and motility in gastric cancer. (2014) Oncotarget. 5:3386-98Read more>>
- Kusuyama J et al. Low intensity pulsed ultrasound (LIPUS) maintains osteogenic potency by the increasedexpression and stability of Nanog through spleen tyrosine kinase (Syk) activation. Cell Signal. 2019 Jun 19;62:109345. Read more>>
- Zhou Y et al. ROCK2 Confers Acquired Gemcitabine Resistance in Pancreatic Cancer Cells by Upregulating Transcription Factor ZEB1. Cancers (Basel). 2019 Nov 27;11(12). pii: E1881. Read more>>
- Xu Jiahui. et al. Temperature and Growth Selection Effects on Proliferation, Differentiation, and Adipogenic Potential of Turkey Myogenic Satellite Cells Through Frizzled-7-Mediated Wnt Planar Cell Polarity Pathway. FRONT PHYSIOL. 2022 May;0:889Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-phospho-ROCK1(Thr455/Ser456) Polyclonal Antibody, Unconjugated (bs-4630R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat heart labeled with Anti-phospho-ROCK1(Thr455/Ser456) Polyclonal Antibody, Unconjugated (bs-4630R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Mouse Cerebrum lysates probed with ROCK1(Thr455+Ser456) Polyclonal Antibody, Unconjugated (bs-4630R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (phospho-ROCK1 (Thr455+Ser456)) polyclonal Antibody, Unconjugated (bs-4630R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-ROCK1 (Thr455+Ser456)) Polyclonal Antibody, Unconjugated (bs-4630R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
MCF-7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with phospho-ROCK1 (Thr455+Ser456) Polyclonal Antibody(bs-4630R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
MCF-7 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with phospho-ROCK1 (Thr455+Ser456) phospho-ROCK1 (Thr455+Ser456) Antibody(bs-4630R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).