Mouse kidney cells probed with Rabbit Anti-AQP2 Polyclonal Antibody (bs-4611R) at 1:50 for 40 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) FITC Conjugated Secondary Antibody.


Formalin-fixed and paraffin embedded mouse kidney tissue labeled with Anti-AQP2 Polyclonal Antibody, unconjugated (bs-4611R) at 1:200 overnight at 37°C followed by labeling Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3) at 1:200, 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei.\n


Mouse kidney lysates probed with Anti-AQP2 Polyclonal Antibody, Unconjugated (bs-4611R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.


Image provided the Independent Validation Program, badge number 029457:Formalin-fixed and paraffin embedded human kidney labeled with Anti-AQP2 Polyclonal Antibody, Unconjugated (bs-4611R) at 1:250 followed by conjugation to the secondary antibody and DAB staining


Image provided by Independent Validation program, badge number 029457:Formalin-fixed and paraffin embedded human breast labeled with Anti-AQP2 Polyclonal Antibody, Unconjugated (bs-4611R) at 1:250 followed by conjugation to the secondary antibody and DAB staining


Rat kidney lysates probed with Aquaporine 2 Polyclonal Antibody, Unconjugated (bs-4611R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Mouse spleen cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with AQP2 Polyclonal Antibody(bs-4611R-AF647)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).


Mouse kidney cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with AQP2 Polyclonal Antibody(bs-4611R-AF647)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).


Kidney lysates probed with AQP2 Polyclonal Antibody, Unconjugated (bs-4611R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.