Host: Rabbit
Target Protein: AQP2
Immunogen Range: 171-271/271
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 359
Swiss Prot: P41181
Source: KLH conjugated synthetic peptide derived from human AQP2.
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 30
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species: Dog
For research use only. Not intended for diagnostic or therapeutic use.
- Lazzeri et al. Endocycle-related tubular cell hypertrophy and progenitor proliferation recover renal function after acute kidney injury. (2018) Nat.Commun. 9:1344Read more>>
- Mr. Aleksandr Vendrov. et al. Renal NOXA1/NOX1 Signaling Regulates Epithelial Sodium Channel and Sodium Retention in Angiotensin II-induced Hypertension. 2021 Oct 29Read more>>
- Shi-Kang Zhou. et al. Fructus Jujubae cooperated with water-expelling members in Shizao decoction alleviated intestinal injury and malignant ascites by modulating gut microbiota and metabolic homeostasis. PHYTOMEDICINE. 2024 Oct;133:155895Read more>>
Mouse kidney cells probed with Rabbit Anti-AQP2 Polyclonal Antibody (bs-4611R) at 1:50 for 40 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) FITC Conjugated Secondary Antibody.
Formalin-fixed and paraffin embedded mouse kidney tissue labeled with Anti-AQP2 Polyclonal Antibody, unconjugated (bs-4611R) at 1:200 overnight at 37°C followed by labeling Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3) at 1:200, 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei.\n
Mouse kidney lysates probed with Anti-AQP2 Polyclonal Antibody, Unconjugated (bs-4611R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.
Image provided the Independent Validation Program, badge number 029457:Formalin-fixed and paraffin embedded human kidney labeled with Anti-AQP2 Polyclonal Antibody, Unconjugated (bs-4611R) at 1:250 followed by conjugation to the secondary antibody and DAB staining
Image provided by Independent Validation program, badge number 029457:Formalin-fixed and paraffin embedded human breast labeled with Anti-AQP2 Polyclonal Antibody, Unconjugated (bs-4611R) at 1:250 followed by conjugation to the secondary antibody and DAB staining
Rat kidney lysates probed with Aquaporine 2 Polyclonal Antibody, Unconjugated (bs-4611R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Mouse spleen cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with AQP2 Polyclonal Antibody(bs-4611R-AF647)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Mouse kidney cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with AQP2 Polyclonal Antibody(bs-4611R-AF647)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Kidney lysates probed with AQP2 Polyclonal Antibody, Unconjugated (bs-4611R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.